Increased phagocytosis capacity of circulating neutrophils in patients on continuous flow ventricular assist device support.
Increased phagocytosis capacity of circulating neutrophils in patients on continuous flow ventricular assist device support.
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在连续流心室辅助装置支持下患者循环中性粒细胞的吞噬能力增加。
DOI：
10.1111/aor.14693
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发表时间：
2023
影响因子：
2.4
通讯作者：
Wu,ZhongjunJ
中科院分区：
文献类型：
--
作者：
Awad,MorcosA;Sun,Wenji;Han,Dong;Griffith,BartleyP;Wu,ZhongjunJ
背景中性粒细胞参与天然免疫反应、吞噬作用和促炎细胞因子释放。循环中性粒细胞的吞噬能力在患者的连续流（CF）心室辅助装置（VAD）还没有得到很好的study.MethodsBlood样本从14例接受CF-VAD植入术收集和分析术前（基线）和术后第3天（POD），7，14和28。使用流式细胞术评估CD 62 L、CD 162和巨噬细胞抗原-1（MAC-1）的表面表达水平和中性粒细胞吞噬能力。使用酶联免疫吸附试验测定血浆中的白细胞介素1（IL 1）、IL 6、IL 8、TNF-α、中性粒细胞弹性蛋白酶和髓过氧化物酶。相对于基线，未植入桥接器械的患者在POD 3时白细胞计数和中性粒细胞弹性蛋白酶升高，在POD 7时恢复正常。术后第3天，中性粒细胞活化水平、IL 6、IL 8和TNF-α升高，并持续升高7-14天。中性粒细胞吞噬能力的升高甚至持续到POD 28。类似的模式，观察到在患者的术前桥device. ConclusionsNeurocytes激活和吞噬细胞的能力增加响应VAD支持，而炎症细胞因子保持升高，长达2周术后。这些结果可能表明，VAD植入eliminates循环中性粒细胞异常抢占吞噬表型。
BackgroundNeutrophils take part in the innate immune response, phagocytosis, and pro‐inflammatory cytokine release. The phagocytic capacity of circulating neutrophils in patients on continuous flow (CF) ventricular assist device (VAD) has not been well studied.MethodsBlood samples from 14 patients undergoing CF‐VAD implantation were collected and analyzed preoperatively (at baseline) and on postoperative days (POD) 3, 7, 14, and 28. Flow cytometry was used to assess the surface expression levels of CD62L, CD162, and macrophage antigen‐1 (MAC‐1) and neutrophil phagocytic capacity. Interleukin 1 (IL1), IL6, IL8, TNF‐α, neutrophil elastase, and myeloperoxidase in plasma were measured using enzyme‐linked immunosorbent assays.ResultsAmong the 14 patients, seven patients had preoperative bridge device support. Relative to baseline, patients with no bridge device had elevated leukocyte count and neutrophil elastase by POD3 which normalized by POD7. Neutrophil activation level, IL6, IL8, and TNF‐α increased by POD3 and sustained elevated levels for 7–14 days postoperatively. Elevated neutrophil phagocytic capacity persisted even until POD28. Similar patterns were observed in patients on a preoperative bridge device.ConclusionsNeutrophil activation and phagocytic capacity increased in response to VAD support, while inflammatory cytokines remain elevated for up to 2 weeks postoperatively. These findings may indicate that VAD implantation elicits circulating neutrophils to an abnormal preemptive phagocytotic phenotype.
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