Articular Chondroprogenitor Cells Maintain Chondrogenic Potential but Fail to Form a Functional Matrix When Implanted Into Muscles of SCID Mice.
Articular Chondroprogenitor Cells Maintain Chondrogenic Potential but Fail to Form a Functional Matrix When Implanted Into Muscles of SCID Mice.
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DOI：
10.1177/1947603514541274
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发表时间：
2014-10
期刊：
影响因子：
2.8
通讯作者：
Archer CW
中科院分区：
文献类型：
--
作者：
Marcus P;De Bari C;Dell'Accio F;Archer CW
关节软骨是一种复杂的组织，包括表型不同的区域。研究已经确定在未成熟关节软骨的表面区域存在祖细胞群。本研究的目的是确定关节软骨祖细胞的体内可塑性。采用纤维连接蛋白差速贴壁法从牛掌指关节分离软骨细胞。将细胞用PKH 26标记并注射到严重联合免疫缺陷（SCID）小鼠的大腿肌肉中。2周后，解剖肌肉并冷冻切片。切片用番红O染色，并标记sox 9和II型胶原。进行聚合酶链反应分析，以确定可塑性的一些组织特异性标记。全层软骨细胞作为对照。2周后，在所有分析的样品中检测到荧光PKH 26标记的细胞。注射新鲜分离的软骨细胞后出现软骨颗粒。注射克隆和富集的软骨祖细胞群体后，没有检测到明显的颗粒，但发现弥漫性软骨结节，番红O染色和Sox 9区域。还检测到低水平的II型胶原蛋白。聚合酶链反应分析确定了内皮细胞标志物PECAM-1的存在下，在一个克隆细胞系，表现为周围宿主组织的表型可塑性。牛关节软骨祖细胞能够在体内移植后存活，但未能产生一个强大的软骨颗粒，尽管表达sox 9和II型胶原。这表明细胞需要进一步的信号进行软骨分化。
Articular cartilage is a complex tissue comprising phenotypically distinct zones. Research has identified the presence of a progenitor cell population in the surface zone of immature articular cartilage. The aim of the present study was to determine the in vivo plasticity of articular cartilage progenitor. Chondropogenitor cells were isolated from bovine metacarpalphalangeal joints by differential adhesion to fibronectin. Cells were labeled with PKH26 and injected into the thigh muscle of severe-combined immunodeficient (SCID) mice. After 2 weeks, the muscles were dissected and cryosectioned. Sections were stained with safranin O and labeled for sox9 and collagen type II. Polymerase chain reaction analysis was carried out to determine plasticity for a number of tissue-specific markers. Full-depth chondrocytes acted as a control. Fluorescent PKH26 labeled cells were detected after 2 weeks in all samples analyzed. A cartilage pellet was present after injection of freshly isolated chondrocytes. After injection with clonal and enriched populations of chondroprogenitors, no distinct pellet was detected, but diffuse cartilage nodules were found with regions of safranin O staining and Sox9. Low levels of collagen type II were also detected. Polymerase chain reaction analysis identified the presence of the endothelial cell marker PECAM-1 in one clonal cell line, demonstrating phenotypic plasticity into the phenotype of the surrounding host tissues. The bovine articular cartilage progenitor cells were able to survive in vivo postimplantation, but failed to create a robust cartilage pellet, despite expressing sox9 and type II collagen. This suggests the cells require further signals for chondrogenic differentiation.
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影响因子：
11.4
作者：
Lefebvre, V;Li, P;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
--
作者：
Ikeda, T;Kamekura, S;Chung, UI
通讯作者：
Chung, UI
DOI：
10.1016/s1357-2725(02)00301-1
发表时间：
2003-04-01
影响因子：
4
作者：
Archer, CW;Francis-West, P
通讯作者：
Francis-West, P
DOI：
10.1083/jcb.144.5.1069
发表时间：
1999-03-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Zhu Y;Oganesian A;Keene DR;Sandell LJ
通讯作者：
Sandell LJ
影响因子：
7
作者：
Lefebvre, V;Behringer, RR;de Crombrugghe, B
通讯作者：
de Crombrugghe, B