Uninterrupted Expression of CmSIT1 in a Sclerotial Parasite Coniothyrium minitans Leads to Reduced Growth and Enhanced Antifungal Ability.
Uninterrupted Expression of CmSIT1 in a Sclerotial Parasite Coniothyrium minitans Leads to Reduced Growth and Enhanced Antifungal Ability.
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CmSIT1 在菌核寄生虫 Coniothyrium minitans 中的不间断表达导致生长减少和抗真菌能力增强
DOI：
10.3389/fmicb.2017.02208
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发表时间：
2017
影响因子：
5.2
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Sun X;Zhao Y;Jia J;Xie J;Cheng J;Liu H;Jiang D;Fu Y
Coniothyrium minitans 是核盘菌的重要真菌寄生虫。此外，它还产生少量的抗真菌物质。 ZS-1TN1812是一种异常突变体，最初是从T-DNA插入文库中筛选出来的。该突变体表现出异常的生长表型，在PDA平板上双重培养时可以显着抑制核盘菌的生长。将ZS-1TN1812的滤液喷洒在油菜叶片上，显着抑制了核盘菌的侵染，表明该突变体产生的抗真菌物质对油菜叶片有效。耐热抗真菌物质可以特异性抑制核盘菌的生长，但不能显着抑制另一种真菌Colletotrichum higginsianum的生长。然而，C. higginsianum 比 S. sclerotiorum 对蛋白质抗生素更敏感。 ZS-1TN1812 中的 T-DNA 插入激活了 CmSIT1 的表达，CmSIT1 是一种参与铁载体介导的铁转运的基因。还确定突变体 ZS-1TN1812 产生具有高铁水平的菌丝。在野生型菌株ZS-1中，CmSIT1仅在与核盘菌接触时才表达，并且CmSIT1的一致过表达表现出与ZS-1TN1812相似的表型。因此，CmSIT1的激活表达导致抗真菌能力增强，CmSIT1是提高C. minitans防治能力的潜在基因。
Coniothyrium minitans is an important mycoparasite of Sclerotinia sclerotiorum. In addition, it also produces small amounts of antifungal substances. ZS-1TN1812, an abnormal mutant, was originally screened from a T-DNA insertional library. This mutant showed abnormal growth phenotype and could significantly inhibit the growth of S. sclerotiorum when dual-cultured on a PDA plate. When spraying the filtrate of ZS-1TN1812 on the leaves of rapeseed, S. sclerotiorum infection was significantly inhibited, suggesting that the antifungal substances produced by this mutant were effective on rapeseed leaves. The thermo-tolerant antifungal substances could specifically suppress the growth of S. sclerotiorum, but could not significantly suppress the growth of another fungus, Colletotrichum higginsianum. However, C. higginsianum was more sensitive to proteinous antibiotics than S. sclerotiorum. The T-DNA insertion in ZS-1TN1812 activated the expression of CmSIT1, a gene involved in siderophore-mediated iron transport. It was also determined that mutant ZS-1TN1812 produced hypha with high iron levels. In the wild-type strain ZS-1, CmSIT1 was expressed only when in contact with S. sclerotiorum, and consistent overexpression of CmSIT1 showed similar phenotypes as ZS-1TN1812. Therefore, activated expression of CmSIT1 leads to the enhanced antifungal ability, and CmSIT1 is a potential gene for improving the control ability of C. minitans.
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影响因子：
16.6
作者：
Cheeseman, Kevin;Ropars, Jeanne;Renault, Pierre;Dupont, Joelle;Gouzy, Jerome;Branca, Antoine;Abraham, Anne-Laure;Ceppi, Maurizio;Conseiller, Emmanuel;Debuchy, Robert;Malagnac, Fabienne;Goarin, Anne;Silar, Philippe;Lacoste, Sandrine;Sallet, Erika;Bensimon, Aaron;Giraud, Tatiana;Brygoo, Yves
通讯作者：
Brygoo, Yves
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
3.1
作者：
Hissen, AHT;Wan, ANC;Moore, MM
通讯作者：
Moore, MM
影响因子：
11.9
作者：
Haas H
通讯作者：
Haas H
影响因子：
14.8
作者：
Kelley, Lawrence A.;Sternberg, Michael J. E.
通讯作者：
Sternberg, Michael J. E.