Mapping astrogliosis in the individual human brain using multidimensional MRI.
Mapping astrogliosis in the individual human brain using multidimensional MRI.
复制标题
DOI：
10.1093/brain/awac298
复制
发表时间：
2023-03-01
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
尽管星形胶质细胞在脑梗塞、神经退行性疾病、创伤性脑损伤和感染等多种疾病的反应中起着重要的作用，但目前尚无非侵入性的成像方法可用于中枢神经系统的星形胶质细胞增生症的评估或定位。多维MRI是一种越来越广泛使用的成像方式，它通过同时探测松弛(T1和T2)和扩散机制来最大化编码的化学和微结构信息量。在这里，我们利用这种想象模式的精致敏感性来得出星形胶质细胞增生症的特征，并使用机器学习在个体水平上将其从标准大脑中分离出来。我们研究了7名遭受冲击伤的受试者和7名对照脑供者的体外大脑皮质组织标本，这些受试者遭受了冲击伤，导致了无其他类型神经病理异常的瘢痕边缘形成的星形胶质细胞增生症。通过进行死后放射学和组织病理学的联合研究，我们发现星形胶质细胞增生症引起的微结构和化学变化被多维MRI强有力地检测到，这可以归因于星形胶质细胞增生症，因为在研究中的任何病例中都没有观察到轴突损伤、脱髓鞘或相互作用。重要的是，我们证明了没有一维的T1、T2或弥散磁共振测量可以解开由这种神经病理引起的微观改变。基于这些发现，我们开发了一种受试者内部的异常检测程序，该程序在体外生成基于磁共振的星形胶质细胞增生生物标记物地图，这些地图与共同登记的胶质纤维酸性蛋白沉积增加的组织学图像显著且强烈相关(扩散-T2、扩散-T1和T1-T2多维数据集，分别为r=0.856，P<；0.0001；r=0.789，P<；0.0001；r=0.793，P<；0.0001)。我们的研究结果阐明了星形胶质细胞增生症的MRI信号反应的基础，以及在个体水平上检测反应性星形胶质细胞的高空间敏感性和特异性，如果在体内复制，将显著影响损伤、疾病、修复和衰老的神经成像研究，其中星形胶质细胞增生症迄今在放射学上是一个不可见的过程。星形胶质细胞增生症可以非侵袭性地观察吗？本贾米尼等人。使用机器学习和多维核磁共振成像，并在个人学科水平上制作冲击波诱导的星形胶质细胞增生症的地图。这种方法可以用来研究损伤、疾病、修复和衰老，在这些过程中，星形胶质细胞增生症以前在放射学上是一个看不见的过程。
There are currently no non-invasive imaging methods available for astrogliosis assessment or mapping in the central nervous system despite its essential role in the response to many disease states, such as infarcts, neurodegenerative conditions, traumatic brain injury and infection. Multidimensional MRI is an increasingly employed imaging modality that maximizes the amount of encoded chemical and microstructural information by probing relaxation (T1 and T2) and diffusion mechanisms simultaneously. Here, we harness the exquisite sensitivity of this imagining modality to derive a signature of astrogliosis and disentangle it from normative brain at the individual level using machine learning. We investigated ex vivo cerebral cortical tissue specimens derived from seven subjects who sustained blast-induced injuries, which resulted in scar-border forming astrogliosis without being accompanied by other types of neuropathological abnormality, and from seven control brain donors. By performing a combined post-mortem radiology and histopathology correlation study we found that astrogliosis induces microstructural and chemical changes that are robustly detected with multidimensional MRI, and which can be attributed to astrogliosis because no axonal damage, demyelination or tauopathy were histologically observed in any of the cases in the study. Importantly, we showed that no one-dimensional T1, T2 or diffusion MRI measurement can disentangle the microscopic alterations caused by this neuropathology. Based on these findings, we developed a within-subject anomaly detection procedure that generates MRI-based astrogliosis biomarker maps ex vivo, which were significantly and strongly correlated with co-registered histological images of increased glial fibrillary acidic protein deposition (r = 0.856, P < 0.0001; r = 0.789, P < 0.0001; r = 0.793, P < 0.0001, for diffusion-T2, diffusion-T1 and T1–T2 multidimensional data sets, respectively). Our findings elucidate the underpinning of MRI signal response from astrogliosis, and the demonstrated high spatial sensitivity and specificity in detecting reactive astrocytes at the individual level, and if reproduced in vivo, will significantly impact neuroimaging studies of injury, disease, repair and aging, in which astrogliosis has so far been an invisible process radiologically. Can astrogliosis be viewed non-invasively? Benjamini et al. employ machine learning with multidimensional MRI and produce maps of blast-induced astrogliosis at the individual subject level. The method could be used to study injury, disease, repair and ageing, in which astrogliosis has previously been an invisible process radiologically.
登录
查看更多内容
影响因子：
5.7
作者：
Benjamini D;Basser PJ
通讯作者：
Basser PJ
影响因子：
3.5
作者：
Budde MD;Annese J
通讯作者：
Annese J
影响因子：
2.9
作者：
Chary, Karthik;Nissi, Mikko J.;Grohn, Olli
通讯作者：
Grohn, Olli
影响因子：
3.1
作者：
Benjamini, Dan;Bouhrara, Mustapha;Basser, Peter J.
通讯作者：
Basser, Peter J.
星形胶质细胞疤痕形成有助于中枢神经系统轴突再生。
影响因子：
64.8
作者：
Anderson MA;Burda JE;Ren Y;Ao Y;O'Shea TM;Kawaguchi R;Coppola G;Khakh BS;Deming TJ;Sofroniew MV
通讯作者：
Sofroniew MV