Generation of a functional liver tissue mimic using adipose stromal vascular fraction cell-derived vasculatures.
Generation of a functional liver tissue mimic using adipose stromal vascular fraction cell-derived vasculatures.
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DOI：
10.1038/srep02141
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发表时间：
2013
影响因子：
4.6
通讯作者：
Boyd, N. L.
中科院分区：
文献类型：
--
作者：
Nunes, S. S.;Maijub, J. G.;Krishnan, L.;Ramakrishnan, V. M.;Clayton, L. R.;Williams, S. K.;Hoying, J. B.;Boyd, N. L.
细胞植入治疗的主要挑战之一是促进植入细胞与宿主之间微循环的整合。我们使用脂肪来源的基质血管部分（SVF）细胞使人肝细胞（HepG2）植入物血管化。我们假设SVF细胞会通过血管组装和与宿主脉管系统的融合形成功能性微循环。最初，我们评估了新分离和培养的SVF细胞形成的新生血管的范围和特征，发现新分离的细胞具有更高的血管化潜力。生成含有新鲜SVF和HepG2细胞的3D植入物，形成HepG2细胞与微血管网络缠绕的组织。仅在SVF血管化的植入物中，通过全身血管内注射将HepG2细胞分离标记LDL传递，这表明SVF细胞衍生的血管可以有效地与宿主血管结合，并与实质细胞结合，形成功能性组织模拟物。
One of the major challenges in cell implantation therapies is to promote integration of the microcirculation between the implanted cells and the host. We used adipose-derived stromal vascular fraction (SVF) cells to vascularize a human liver cell (HepG2) implant. We hypothesized that the SVF cells would form a functional microcirculation via vascular assembly and inosculation with the host vasculature. Initially, we assessed the extent and character of neovasculatures formed by freshly isolated and cultured SVF cells and found that freshly isolated cells have a higher vascularization potential. Generation of a 3D implant containing fresh SVF and HepG2 cells formed a tissue in which HepG2 cells were entwined with a network of microvessels. Implanted HepG2 cells sequestered labeled LDL delivered by systemic intravascular injection only in SVF-vascularized implants demonstrating that SVF cell-derived vasculatures can effectively integrate with host vessels and interface with parenchymal cells to form a functional tissue mimic.
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影响因子：
1.2
作者：
Cervelli, Valerio;Gentile, Pietro;Orlandi, Augusto
通讯作者：
Orlandi, Augusto
DOI：
10.1152/ajpheart.00735.2011
发表时间：
2012-02-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.1
作者：
Chiu, Loraine L. Y.;Iyer, Rohin K.;Radisic, Milica
通讯作者：
Radisic, Milica
影响因子：
--
作者：
Kedem, A;Perets, A;Cohen, S
通讯作者：
Cohen, S