Proteomic profiling of human islets collected from frozen pancreata using laser capture microdissection.
Proteomic profiling of human islets collected from frozen pancreata using laser capture microdissection.
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DOI：
10.1016/j.jprot.2016.09.002
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发表时间：
2017-01-06
影响因子：
3.3
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Zhang L;Lanzoni G;Battarra M;Inverardi L;Zhang Q
1型糖尿病（T1D）的病因仍然难以捉摸。酶促分离培养（EIC）胰岛由于分离过程的压力，不能完全反映体内胰岛的天然蛋白质组成和疾病过程。为了在接近自然环境的条件下研究胰岛蛋白组成，我们对EIC胰岛进行了蛋白质组学分析，并从三具尸体供体的新鲜冷冻胰腺切片中激光捕获微解剖（LCM）人胰岛和腺泡组织。从LCM胰岛和腺泡组织的6个胰岛当量（IEQ）中分别鉴定出1104和706个蛋白。LCM胰岛的蛋白质组学图谱在尸体供者体内和尸体供者之间是可重复的。内分泌激素仅在LCM胰岛中检测到，而催化酶在腺泡组织中显著富集。此外，LCM和EIC胰岛蛋白质组之间存在高重叠（984个蛋白）和相似的功能分布，只是EIC胰岛具有更多的腺泡污染物和应激相关信号转导活性蛋白。LCM胰岛、LCM腺泡组织和EIC胰岛蛋白质组的比较表明，LCM结合蛋白质组学方法可以准确、公正地分析冷冻胰岛蛋白质组。这为T1D进展过程中人类胰岛蛋白组学研究铺平了道路。
The etiology of Type 1 Diabetes (T1D) remains elusive. Enzymatically isolated and cultured (EIC) islets cannot fully reflect the natural protein composition and disease process of in vivo islets, because of the stress from isolation procedures. In order to study islet protein composition in conditions close to the natural environment, we performed proteomic analysis of EIC islets, and laser capture microdissected (LCM) human islets and acinar tissue from fresh-frozen pancreas sections of three cadaveric donors. 1104 and 706 proteins were identified from 6 islets equivalents (IEQ) of LCM islets and acinar tissue, respectively. The proteomic profiles of LCM islets were reproducible within and among cadaveric donors. The endocrine hormones were only detected in LCM islets, whereas catalytic enzymes were significantly enriched in acinar tissue. Furthermore, high overlap (984 proteins) and similar function distribution were found between LCM and EIC islets proteomes, except that EIC islets had more acinar contaminants and stress-related signal transducer activity proteins. The comparison among LCM islets, LCM acinar tissue and EIC islets proteomes indicates that LCM combined with proteomic methods enables accurate and unbiased profiling of islet proteome from frozen pancreata. This paves the way for proteomic studies on human islets during the progression of T1D.
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影响因子：
3.7
作者：
Marselli L;Thorne J;Dahiya S;Sgroi DC;Sharma A;Bonner-Weir S;Marchetti P;Weir GC
通讯作者：
Weir GC
DOI：
10.1038/npp.2008.172
发表时间：
2009-01
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
8
作者：
Campbell-Thompson, Martha;Wasserfall, Clive;Kaddis, John;Albanese-O'Neill, Anastasia;Staeva, Teodora;Nierras, Concepcion;Moraski, Jayne;Rowe, Patrick;Gianani, Roberto;Eisenbarth, George;Crawford, James;Schatz, Desmond;Pugliese, Alberto;Atkinson, Mark
通讯作者：
Atkinson, Mark
影响因子：
1.2
作者：
Brackeva B;Kramer G;Vissers JP;Martens GA
通讯作者：
Martens GA
影响因子：
2.7
作者：
Kutlu, Burak;Burdick, David;Baxter, David;Rasschaert, Joanne;Flamez, Daisy;Eizirik, Decio L.;Welsh, Nils;Goodman, Nathan;Hood, Leroy
通讯作者：
Hood, Leroy