Single-cell RNA-seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.).
Single-cell RNA-seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.).
复制标题
DOI：
10.1111/pbi.13656
复制
发表时间：
2021-11
影响因子：
13.8
通讯作者：
Hong Y
中科院分区：
文献类型：
--
作者：
Liu H;Hu D;Du P;Wang L;Liang X;Li H;Lu Q;Li S;Liu H;Chen X;Varshney RK;Hong Y
单细胞RNA-seq(scRNA-seq)已被认为是描述人类细胞转录组的有力工具，但该技术尚未在植物细胞中广泛应用。在这里，我们描述了一个强大的花生叶片原生质体细胞分离系统的成功开发。根据已报道的标记基因，应用scRNA-seq将6815个单个细胞分为8个细胞团。此外，利用伪时间分析描述了不同细胞类型转录因子在叶片生长过程中的发育轨迹和相互作用网络。这一轨迹使得重新研究叶肉和表皮的原基驱动的发育过程成为可能。这些结果表明，栅栏细胞可能分化为海绵状细胞，而表皮细胞起源早于原基。随后，开发的方法集成了多种技术来有效验证scRNA-seq结果，以获得均一的细胞群体。根据获得的scRNA-seq值，几种转录因子的表达水平与表皮个体发育密切相关。此外，定位于细胞核的花生AHL23(AT-Hook基序核定位蛋白23)通过调节植物激素途径在拟南芥中异地表达时，促进了叶片的生长。总之，我们的研究表明，应用scRNA-seq可以为花生叶片的细胞分化提供新的假设。我们相信，这种方法将使异源四倍体花生和其他植物的叶片细胞功能研究取得重大进展。
Single‐cell RNA‐seq (scRNA‐seq) has been highlighted as a powerful tool for the description of human cell transcriptome, but the technology has not been broadly applied in plant cells. Herein, we describe the successful development of a robust protoplast cell isolation system in the peanut leaf. A total of 6,815 single cells were divided into eight cell clusters based on reported marker genes by applying scRNA‐seq. Further, a pseudo‐time analysis was used to describe the developmental trajectory and interaction network of transcription factors (TFs) of distinct cell types during leaf growth. The trajectory enabled re‐investigation of the primordium‐driven development processes of the mesophyll and epidermis. These results suggest that palisade cells likely differentiate into spongy cells, while the epidermal cells originated earlier than the primordium. Subsequently, the developed method integrated multiple technologies to efficiently validate the scRNA‐seq result in a homogenous cell population. The expression levels of several TFs were strongly correlated with epidermal ontogeny in accordance with obtained scRNA‐seq values. Additionally, peanut AHL23 (AT‐HOOK MOTIF NUCLEAR LOCALIZED PROTEIN 23), which is localized in nucleus, promoted leaf growth when ectopically expressed in Arabidopsis by modulating the phytohormone pathway. Together, our study displays that application of scRNA‐seq can provide new hypotheses regarding cell differentiation in the leaf blade of Arachis hypogaea. We believe that this approach will enable significant advances in the functional study of leaf blade cells in the allotetraploid peanut and other plant species.
登录
查看更多内容
DOI：
10.1093/plcell/koaa060
发表时间：
2021-05-05
期刊：
The Plant cell
影响因子：
--
作者：
Kim JY;Symeonidi E;Pang TY;Denyer T;Weidauer D;Bezrutczyk M;Miras M;Zöllner N;Hartwig T;Wudick MM;Lercher M;Chen LQ;Timmermans MCP;Frommer WB
通讯作者：
Frommer WB
通过单细胞 RNA 测序对气孔谱系细胞发育进行全局动态分子分析
影响因子：
27.5
作者：
Liu, Zhixin;Zhou, Yaping;Sun, Xuwu
通讯作者：
Sun, Xuwu
全转录组测序提供了对花生 (Arachishypogaea L.) 果果性的深入了解
影响因子：
13.8
作者：
Chen, Xiaoping;Yang, Qingli;Yu, Shanlin
通讯作者：
Yu, Shanlin
影响因子：
27.5
作者：
Jia, Qi-Shi;Zhu, Jun;Yang, Zhong-Nan
通讯作者：
Yang, Zhong-Nan
影响因子：
64.5
作者：
Nayfach S;Pollard KS
通讯作者：
Pollard KS