Flight and seizure motor patterns in Drosophila mutants: simultaneous acoustic and electrophysiological recordings of wing beats and flight muscle activity.
Flight and seizure motor patterns in Drosophila mutants: simultaneous acoustic and electrophysiological recordings of wing beats and flight muscle activity.
复制标题
DOI：
10.3109/01677063.2014.957827
复制
发表时间：
2014-09
影响因子：
1.9
通讯作者：
Wu CF
中科院分区：
文献类型：
--
作者：
Iyengar A;Wu CF
系留苍蝇可以研究飞行控制的生物力学和电生理学。我们对间接飞行肌肉（背侧纵向肌肉，DLMa）中的尖峰进行了微电极记录，并通过麦克风信号对翅膀拍动频率（WBF）进行了声学分析。直接和间接飞行肌肉的同时电生理记录在技术上具有挑战性；然而，WBF 被认为反映了与直接飞行肌肉子集（包括 m1b 肌肉）中的尖峰的一对一关系。因此，我们的方法能够对支配直接和间接飞行肌肉子集的运动神经元的电活动的时间特征的变化进行系统的突变分析。在这里，我们报告了特定离子通道破坏对肌源性 DLM 的尖峰活动（以〜5 Hz 发射）和相应的翅膀拍打频率（〜200 Hz）的影响。我们检查了以下突变体：1）电压门控 Ca2+ 通道（cacophony，cac），2）Ca2+ 激活 K+ 通道（slowpoke，slo）和 3）电压门控 K+ 通道（Shaker，Sh）及其辅助亚基（Hyperkinetic，Hk 和 quiver，qvr）。我们发现 cac 和 slo 突变体对吹气的反应启动飞行受到严重干扰。然而，一旦启动，slo 飞行基本上没有改变，而 cac 则显示 DLM 发射率和 WBF 受到干扰。尽管 WBF 增加，Sh、Hk 和 qvr 突变体仍能够维持正常的 DLM 放电率。值得注意的是，辅助亚基 Hk 和 qvr 的缺陷可能会导致不同的后果，即 DLM 放电节律性被破坏，而在 Sh 中没有观察到。我们对直接和间接飞行肌肉活动的突变分析表明，两种运动活动模式可能会被特定的离子通道突变独立修改，并且这种方法可以扩展到其他双翅目物种和其他运动程序，例如电惊厥刺激诱发的癫痫发作。
Tethered flies allow studies of biomechanics and electrophysiology of flight control. We performed microelectrode recordings of spikes in an indirect flight muscle (the dorsal longitudinal muscle, DLMa) coupled with acoustic analysis of wing beat frequency (WBF) via microphone signals. Simultaneous electrophysiological recording of direct and indirect flight muscles has been technically challenging; however, the WBF is thought to reflect in a one-to-one relationship with spiking in a subset of direct flight muscles, including muscle m1b. Therefore, our approach enables systematic mutational analysis for changes in temporal features of electrical activity of motor neurons innervating subsets of direct and indirect flight muscles. Here we report the consequences of specific ion channel disruptions on the spiking activity of myogenic DLMs (firing at ∼5 Hz) and the corresponding wing beat frequency (∼200 Hz). We examined mutants of: 1) voltage-gated Ca2+ channels (cacophony, cac), 2) Ca2+-activated K+ channels (slowpoke, slo), and 3) voltage-gated K+ channels (Shaker, Sh) and their auxiliary subunits (Hyperkinetic, Hk and quiver, qvr). We found flight initiation in response to an air puff was severely disrupted in both cac and slo mutants. However, once initiated, slo flight was largely unaltered, whereas cac displayed disrupted DLM firing rates and WBF. Sh, Hk, and qvr mutants were able to maintain normal DLM firing rates, despite increased WBF. Notably, defects in the auxiliary subunits Hk and qvr could lead to distinct consequences, i.e. disrupted DLM firing rhythmicity, not observed in Sh. Our mutant analysis of direct and indirect flight muscle activities indicates that the two motor activity patterns may be independently modified by specific ion channel mutations, and that this approach can be extended to other dipteran species and additional motor programs, such as electroconvulsive stimulation-induced seizures.
登录
查看更多内容
DOI：
10.1523/jneurosci.0502-11.2011
发表时间：
2011-08-03
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dean T;Xu R;Joiner W;Sehgal A;Hoshi T
通讯作者：
Hoshi T
DOI：
10.1007/bf00656637
发表时间：
1977-01-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY
影响因子：
--
作者：
EWING, AW
通讯作者：
EWING, AW
影响因子：
1.2
作者：
Babcock, Daniel T.;Ganetzky, Barry
通讯作者：
Ganetzky, Barry
DOI：
10.1007/bf00195964
发表时间：
1992-08-01
影响因子：
2.1
作者：
ENGEL, JE;WU, CF
通讯作者：
WU, CF
DOI：
10.1073/pnas.83.21.8415
发表时间：
1986-11-01
影响因子：
11.1
作者：
ELKINS, T;GANETZKY, B;WU, CF
通讯作者：
WU, CF