Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-γ, but not transforming growth factor-β dependent.
Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-γ, but not transforming growth factor-β dependent.
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DOI：
10.1111/j.1365-2222.2010.03545.x
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Li XM
中科院分区：
文献类型：
--
作者：
Srivastava K;Zhang T;Yang N;Sampson H;Li XM
慢性过敏性哮喘是Th 2偏向免疫状态的结果。目前的哮喘治疗控制了一些患者的症状，但尚未建立持久的治疗。ASHMI™是一种中草药配方，在持续性中度至重度哮喘患者的对照试验中改善了症状和肺功能，并降低了Th 2反应。我们在持续性哮喘的小鼠模型中评估了治疗后ASHMI™有益效果的持续性以及这种效果的免疫学机制。用卵清蛋白（OVA）腹腔内致敏的BALB/c小鼠每周接受3次腹腔内OVA激发以诱导气道高反应性（AHR）和炎症（OVA小鼠）。将另外的OVA小鼠用ASHMI™（OVA/ASHMI™）或水（OVA/Sham）处理4周，然后立即激发和治疗后8周激发。在其他实验中，OVA小鼠接受ASHMI™处理，同时中和IFN-γ或TGF-β。治疗后8周测定对气道反应、细胞因子和OVA特异性IgE水平的影响。在治疗前，OVA小鼠在OVA激发后表现出AHR和肺嗜酸性粒细胞炎症，其在完成ASHMI™治疗后几乎立即完全消退，并且在治疗后长达8周的OVA再激发后没有再次发生。降低的过敏原特异性IgE和Th 2细胞因子水平，以及增加的IFN-γ水平也持续治疗后至少8周。在治疗期间，通过中和IFN-γ而不是TGF-β消除了ASHMI™效应。ASHMI™诱导对抗原诱导的炎症和AHR的持久治疗后耐受。IFN-γ是ASHMI™效应中的关键因素。
Chronic allergic asthma is the result of a Th2- biased immune status. Current asthma therapies control symptoms in some patients, but a long lasting therapy has not been established. ASHMI™, a Chinese herbal formula improved symptoms and lung function, and reduced Th2 responses in a controlled trial of patients with persistent moderate to severe asthma. We evaluated the persistence of ASHMI™ beneficial effects following therapy in a murine model of persistent asthma and the immunological mechanisms underlying such effects. BALB/c mice sensitized intraperitoneally with ovalbumin (OVA) received 3 weekly intratracheal OVA challenges to induce airway hyperreactivity (AHR) and inflammation (OVA mice). Additional OVA mice were treated with ASHMI™ (OVA/ASHMI™) or water (OVA/Sham) for 4 weeks, and then challenged immediately and eight weeks post-therapy. In other experiments OVA mice received ASHMI™ treatment with concomitant neutralization of IFN-γ or TGF-β. Effects on airway responses, cytokine and OVA-specific IgE levels were determined 8 weeks post-therapy. Prior to treatment, OVA mice exhibited AHR and pulmonary eosinophilic inflammation following OVA challenge, which was almost completely resolved immediately after completing treatment with ASHMI™ and did not re-occur following OVA re-challenge up to 8 wks post-therapy. Reduced allergen-specific IgE and Th2 cytokine levels, and increased IFN-γ levels also persisted at least 8 wks post-therapy. ASHMI™ effects were eliminated by neutralization of IFN-γ, but not TGF-β, during therapy. ASHMI™ induced long-lasting post-therapy tolerance to antigen-induced inflammation and AHR. IFN-γ is a critical factor in ASHMI™ effects.
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DOI：
10.1097/aci.0b013e328329226f
发表时间：
2009-04
影响因子：
2.8
作者：
Li XM
通讯作者：
Li XM
DOI：
10.1164/rccm.200304-499oc
发表时间：
2004-01-01
影响因子：
24.7
作者：
Guerra, S;Lohman, IC;Wright, AL
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Wright, AL
影响因子：
14.2
作者：
Lemanske, RF
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Lemanske, RF
影响因子：
14.2
作者：
Karwot, Roman;Maxeiner, Joachim H.;Finotto, Susetta
通讯作者：
Finotto, Susetta
DOI：
10.1152/ajplung.90275.2008
发表时间：
2009-02-01
影响因子：
4.9
作者：
Lim, Dae Hyun;Cho, Jae Youn;Broide, David H.
通讯作者：
Broide, David H.