Long-wavelength optical coherence tomography at 1.7 microm for enhanced imaging depth.
Long-wavelength optical coherence tomography at 1.7 microm for enhanced imaging depth.
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DOI：
10.1364/oe.16.019712
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发表时间：
2008-11-24
期刊：
影响因子：
3.8
通讯作者：
Yun SH
中科院分区：
文献类型：
--
作者：
Sharma U;Chang EW;Yun SH
在光学相干层析成像（OCT）中，样品中的多次散射对在更深的穿透深度处获得有意义的结构信息提出了显著的限制。先前的研究表明，与广泛使用的1.3 µm左右的波长相比，1.7 µm左右的光谱区域可能会在生物组织中表现出降低的散射系数。为了研究这个长波长区域，我们开发了一种波长为1.7 µm的波长扫描激光器，并首次在该光谱范围内进行了OCT或光学频域成像（OFDI）。所构建的激光器能够在160 nm范围内提供1.59至1.75 µm的宽调谐范围。当激光器在10.9 kHz的重复频率和12.3 mW的平均输出功率下以超过95 nm的减小的调谐范围操作时，对外直接投资成像系统表现出约100 dB的灵敏度以及分别为24 µm和14 µm的轴向和横向分辨率。我们使用1.3 µm和1.7 µm OFDI系统对几个体模和生物样本进行成像，发现在大多数（如果不是所有样本的话）1.7 µm波长下，深度相关的信号衰减率显著降低。我们的研究结果表明，这种成像窗口可以提供一个优势，通过增加穿透深度，以及在更深的穿透深度，否则多个散射光子占主导地位的弹道光子增强图像对比度较短的波长。
Multiple scattering in a sample presents a significant limitation to achieve meaningful structural information at deeper penetration depths in optical coherence tomography (OCT). Previous studies suggest that the spectral region around 1.7 µm may exhibit reduced scattering coefficients in biological tissues compared to the widely used wavelengths around 1.3 µm. To investigate this long-wavelength region, we developed a wavelength-swept laser at 1.7 µm wavelength and conducted OCT or optical frequency domain imaging (OFDI) for the first time in this spectral range. The constructed laser is capable of providing a wide tuning range from 1.59 to 1.75 µm over 160 nm. When the laser was operated with a reduced tuning range over 95 nm at a repetition rate of 10.9 kHz and an average output power of 12.3 mW, the OFDI imaging system exhibited a sensitivity of about 100 dB and axial and lateral resolution of 24 µm and 14 µm, respectively. We imaged several phantom and biological samples using 1.3 µm and 1.7 µm OFDI systems and found that the depth-dependent signal decay rate is substantially lower at 1.7 µm wavelength in most, if not all samples. Our results suggest that this imaging window may offer an advantage over shorter wavelengths by increasing the penetration depths as well as enhancing image contrast at deeper penetration depths where otherwise multiple scattered photons dominate over ballistic photons.
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