NF-κB inhibition facilitates the establishment of cell lines that chronically produce human T-lymphotropic virus type 1 viral particles.
NF-κB inhibition facilitates the establishment of cell lines that chronically produce human T-lymphotropic virus type 1 viral particles.
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NF-κB 抑制促进长期产生人类 T 淋巴细胞病毒 1 型病毒颗粒的细胞系的建立。
DOI：
10.1128/jvi.02961-13
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发表时间：
2014
影响因子：
5.4
通讯作者：
Giam,Chou-Zen
中科院分区：
文献类型：
--
作者：
Zahoor,MuhammadAtif;Philip,Subha;Zhi,Huijun;Giam,Chou-Zen
大多数感染人类T淋巴细胞病毒1型(HTLV-1)的HeLa和SupT1细胞在感染HTLV-1或转导HTLV-1tax基因后立即停止增殖并衰老。TAX引起的细胞衰老反应是由高激活的NF-κB引起的，并由细胞周期蛋白依赖性激酶抑制剂p21CIP1/WAF1和p27KIP1介导。当NF-κB活性被抗降解形式的IκBα，ΔN-IκBα阻断时，税收诱导的衰老被避免。在这里，我们表明，通过抑制核因子-κB通过ΔN-IκBα的表达，允许人骨肉瘤细胞系的细胞被HTLV-1慢性感染。可以很容易地建立和分离稳定的HTLV-1产生的HOS细胞克隆。这些克隆在培养中继续增殖，持续表达Tax、Rex、Gag和Env蛋白，并在共培养后很容易地将HTLV-1传递给未成熟的HOS、SupT1和Jurkat T报告细胞系。由于HOS细胞附着在培养板上，悬液中感染的T细胞可以很容易地收集和鉴定。通过抑制核因子-κB在细胞培养中建立慢性和生产性的HTLV-1感染，为深入研究HTLV-1复制的分子事件和病毒基因的作用机制提供了有用的手段。该系统为研究HTLV-1在细胞培养中的复制奠定了基础。
Most human T-lymphotropic virus type 1 (HTLV-1)-infected HeLa and SupT1 cells cease proliferation and become senescent immediately after infection by HTLV-1 or transduction of the HTLV-1taxgene. The cellular senescence response triggered by Tax is caused by hyperactivated NF-κB and mediated by cyclin-dependent kinase inhibitors, p21CIP1/WAF1and p27KIP1. When NF-κB activity is blocked by a degradation-resistant form of IκBα, ΔN-IκBα, Tax-induced senescence is averted. Here, we show that NF-κB inhibition through the expression of ΔN-IκBα allows cells of a human osteosarcoma (HOS) cell line to be chronically infected by HTLV-1. Stable HTLV-1-producing HOS cell clones can be readily established and isolated. These clones continue to proliferate in culture; express Tax, Rex, Gag, and Env proteins persistently; and transmit HTLV-1 to naive HOS, SupT1, and Jurkat T reporter cell lines readily after cocultivation. As HOS cells are adherent to culture plates, infected T cells in suspension can be easily collected and characterized. The ease with which chronic and productive HTLV-1 infection can be established in cell culture through inhibition of NF-κB affords a useful means to examine in depth the molecular events of HTLV-1 replication and the mechanisms of action of viral genes.IMPORTANCEThis paper describes a system for establishing cell lines that can be productively infected by human T-lymphotropic virus type 1 (HTLV-1) and can spread HTLV-1 to susceptible cells. Such a system can facilitate the study of HTLV-1 replication in cell culture.
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影响因子：
6.1
作者：
Takagi,M;Franco-Saenz,R;Mulrow,PJ
通讯作者：
Mulrow,PJ
影响因子：
15.9
作者：
ATARASHI, K;MULROW, PJ;FRANCOSAENZ, R
通讯作者：
FRANCOSAENZ, R
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影响因子：
4.1
作者：
J. Mauger;J. Poggioli;F. Guesdon;M. Claret
通讯作者：
M. Claret
影响因子：
56.9
作者：
CURRIE, MG;GELLER, DM;NEEDLEMAN, P
通讯作者：
NEEDLEMAN, P
ANF 抑制 ANG II 刺激醛固酮分泌，但不抑制 ANG II 产生 [Ca2 ]、瞬态
DOI：
10.1097/00005344-198611000-00199
发表时间：
1986
期刊：
Journal of hypertension. Supplement : official journal of the International Society of Hypertension
影响因子：
--
作者：
W. Apfeldorf;P. Barrett;H. Rasmussen
通讯作者：
H. Rasmussen