An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis.

An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis.
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乙烯反应因子 (MxERF4) 在小金海棠中充当 Fe 获取的抑制因子。

DOI:
10.1038/s41598-018-19518-4
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发表时间:
2018-01-18
期刊:
影响因子:
4.6
通讯作者:
Wang Y
Wang Y
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Liu W;Wu T;Li Q;Zhang X;Xu X;Li T;Han Z;Wang Y

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铁(Fe)是植物的必需元素;然而,它的可用性是有限的,因为它在土壤中形成不溶性络合物。因此,植物已经形成了适应低铁条件的机制。我们证明,乙烯参与缺铁诱导的生理反应,在小金海棠,并描述了MxERF 4作为蛋白质-蛋白质相互作用的伴侣与MxFIT转录因子,这是参与缺铁反应的鉴定。此外,我们证明,MxERF 4作为MxFIT相互作用的合作伙伴,抑制铁转运蛋白MxIRT 1的表达,通过直接结合到其启动子,需要的MxERF 4蛋白的ERF 3基序。抑制M.利用病毒介导的基因沉默技术,在小金海棠中诱导MxIRT 1基因表达增加。两者合计,结果表明一个镇压机制,乙烯启动Fe缺乏反应,然后抑制反应,这可能需要通过MxERF 4的作用短暂抑制Fe的收购。
Iron (Fe) is an essential element for plants; however, its availability is limited as it forms insoluble complexes in the soil. Consequently, plants have developed mechanisms to adapt to low Fe conditions. We demonstrate that ethylene is involved in Fe deficiency-induced physiological responses in Malus xiaojinensis, and describe the identification of MxERF4 as a protein-protein interaction partner with the MxFIT transcription factor, which is involved in the iron deficiency response. Furthermore, we demonstrate that MxERF4 acts as an MxFIT interaction partner to suppresses the expression of the Fe transporter MxIRT1, by binding directly to its promoter, requiring the EAR motif of the MxERF4 protein. Suppression of MxERF4 expression in M. xiaojinensis, using virus induced gene silencing resulted in an increase in MxIRT1 expression. Taken together, the results suggest a repression mechanism, where ethylene initiates the Fe deficiency response, and the response is then dampened, which may require a transient inhibition of Fe acquisition via the action of MxERF4.
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