The LG/J murine strain exhibits near-normal tendon biomechanical properties following a full-length central patellar tendon defect.
The LG/J murine strain exhibits near-normal tendon biomechanical properties following a full-length central patellar tendon defect.
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DOI：
10.1080/03008207.2016.1213247
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发表时间：
2016-11
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
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作者：
需要确定生物学成功标准以改进治疗，识别它们的一种策略是分析肌腱愈合成功和不成功模型的RNA转录组。我们已经描述了MRL/MpJ小鼠品系的特征，并发现中心髌腱损伤后机械结果得到改善。在这项研究中，我们评估了LG/J小鼠品系的愈合，其中包括75%的MRL/MpJ背景，以确定LG/J是否也表现出改善的生物力学性能损伤后，并确定差异表达的转录因子跨C57 BL/6，MRL/MpJ和LG/J菌株在愈合的早期阶段。在16至20周龄的MRL/MpJ、LG/J和C57 BL/6小鼠品系中产生全长中央髌腱（PT）缺损。在术后2、5和8周评估机械性能。使用新型聚类软件程序在损伤后3天、7天和14天评估转录组表达，以评估转录因子的差异表达。术后8周，平均LG/J结构性能分别改善至原始LG/J PT刚度和极限载荷的96.7%和97.2%。我们发现LG/J通过增加与炎症反应和胶原纤维组织有关的转录因子的表达来响应。LG/J应变在8周时恢复到正常的结构特性，并且在每个时间点具有稳定增加的特性。未来的工作将表征细胞群体对损伤的反应，并研究差异表达的转录因子在愈合过程中的作用。
Identifying biological success criteria is needed to improve therapies, and one strategy for identifying them is to analyze the RNA transcriptome for successful and unsuccessful models of tendon healing. We have characterized the MRL/MpJ murine strain and found improved mechanical outcomes following a central patellar tendon injury. In this study, we evaluate the healing of the LG/J murine strain, which comprises 75% of the MRL/MpJ background, to determine if the LG/J also exhibits improved biomechanical properties following injury and to determine differentially expressed transcription factors across the C57BL/6, MRL/MpJ and the LG/J strains during the early stages of healing. A full-length, central patellar tendon (PT) defect was created in 16 to 20 week old MRL/MpJ, LG/J, and C57BL/6 murine strains. Mechanical properties were assessed at 2, 5, and 8 weeks post surgery. Transcriptomic expression was assessed at 3, 7, and 14 days following injury using a novel clustering software program to evaluate differential expression of transcription factors. Average LG/J structural properties improved to 96.7% and 97.2% of native LG/J PT stiffness and ultimate load by 8 weeks post surgery, respectively. We found the LG/J responded by increasing expression of transcription factors implicated in the inflammatory response and collagen fibril organization. The LG/J strain returns to normal structural properties by 8 weeks, with steadily increasing properties at each time point. Future work will characterize the cell populations responding to injury and investigate the role of the differentially expressed transcription factors during healing.
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DOI：
10.1534/g3.113.007302
发表时间：
2013-11-06
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Rai MF;Schmidt EJ;McAlinden A;Cheverud JM;Sandell LJ
通讯作者：
Sandell LJ
影响因子：
6.9
作者：
Rai, Muhammad Farooq;Sandell, Linda J.
通讯作者：
Sandell, Linda J.
影响因子：
3.8
作者：
Bruns, J;Kampen, J;Plitz, W
通讯作者：
Plitz, W
影响因子：
2.8
作者：
Awad, HA;Boivin, GP;Butler, DL
通讯作者：
Butler, DL
RNA-seq数据：实验设计和分析的挑战和建议。
影响因子：
--
作者：
Williams, Alexander G;Thomas, Sean;Wyman, Stacia K;Holloway, Alisha K
通讯作者：
Holloway, Alisha K