Geometry of conjugated double bonds of CLA isomers in a commercial mixture and in their hepatic 20∶4 metabolites
Geometry of conjugated double bonds of CLA isomers in a commercial mixture and in their hepatic 20∶4 metabolites
复制标题
商业混合物及其肝脏 20:4 代谢物中 CLA 异构体共轭双键的几何结构
DOI：
10.1007/s11745-999-0484-3
复制
发表时间：
1999
期刊：
影响因子：
1.9
通讯作者：
C. Ginies
中科院分区：
文献类型：
--
作者：
J. Sebedio;P. Juanéda;S. Grégoire;J. Chardigny;J. ;C. Ginies
给大鼠喂无脂饲料2周。在此期间之后，在维持动物相同饮食的同时，每天给大鼠灌胃180 mg共轭亚油酸（CLA）作为三酰甘油的混合物。对该混合物的气相色谱-质谱（GC-MS）分析，以及肼还原和GC-MS和GC-Fourier变换红外分析，显示存在两种主要的异构体，9 c，11 t-和10 t，12 c-18∶2，伴随着少量的8 t，10 c和11 c，13 t −18∶2异构体。还检测到少量的顺式、顺式和反式、反式共轭异构体。采用反相高效液相色谱法分离肝脂中的总脂肪酸甲酯，并采用银薄层色谱法分离含有20∶4异构体的组分。用肼还原一个含有两个20∶4共轭异构体的谱带，用GC-MS分析得到的单烯为二甲基恶唑啉衍生物。这两个共轭异构体被初步鉴定为5c，8 c，11 c，13 t-20∶4和5c，8 c，12 t，14 c −20∶4。这可能是由于共轭亚油酸混合物中9 c，11 t-和10 t，12 c-18∶2的去饱和和伸长而形成的。
Rats were fed a fat-free diet for 2 wk. After this period, while maintaining the animals on the same diet, the rats were given intragastrically 180 mg per day of a mixture of conjugated linoleic acids (CLA) as triacylglycerols. Gas chromatography-mass spectrometry (GC-MS) analyses of this mixture, as well as hydrazine reduction and GC-MS and GC-Fourier transform infrared analyses of the resulting monoenes, revealed the presence of two major isomers, the 9c, 11t-and the 10t, 12c-18∶2 accompanied by smaller amounts of the 8t, 10c and the 11c,13t−18∶2 isomers. Minor quantities of cis,cis and trans,trans conjugated isomers also were detected. The total fatty acid methyl esters from the liver lipids were fractionated by reversed-phase high-performance liquid chromatography, and the fraction containing the 20∶4 isomers was further fractionated by silver nitrate thin-layer chromatography. A band containing two 20∶4 conjugated isomers was submitted to hydrazine reduction and the resulting monoenes analyzed by GC-MS as dimethyl-oxazoline derivatives. The two conjugated isomers were tentatively identified as 5c,8c,11c,13t–20∶4 and 5c,8c,12t,14c−20∶4. These could be formed by desaturation and elongation of the 9c,11t-and 10t,12c−18∶2 present in the commerical CLA mixture.
登录
查看更多内容
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith
DOI：
--
发表时间：
1997
期刊：
Artery
影响因子：
--
作者：
R. Nicolosi;E. Rogers;D. Kritchevsky;J. Scimeca;P. Huth
通讯作者：
R. Nicolosi;E. Rogers;D. Kritchevsky;J. Scimeca;P. Huth
影响因子：
11.2
作者：
Y. Ha;J. Storkson;M. Pariza
通讯作者：
Y. Ha;J. Storkson;M. Pariza
影响因子：
4.7
作者：
HA, YL;GRIMM, NK;PARIZA, MW
通讯作者：
PARIZA, MW
共轭亚油酸对乳腺形态和生化状态的影响：对降低乳腺癌风险的影响。
DOI：
--
发表时间：
1997
期刊：
Cancer research.
影响因子：
--
作者：
Thompson,H;Zhu,Z;Banni,S;Darcy,K;Loftus,T;Ip,C
通讯作者：
Ip,C