Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers.
Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers.
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DOI：
10.1084/jem.20111449
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发表时间：
2011-11-21
期刊：
影响因子：
--
通讯作者：
Cyster JG
中科院分区：
文献类型：
--
作者：
Wang X;Cho B;Suzuki K;Xu Y;Green JA;An J;Cyster JG
选择性消融小鼠滤泡树突状细胞导致原代滤泡紊乱，B细胞分散出脾生发中心。滤泡树突状细胞（fdc）在初代滤泡和生发中心（GCs）中保留和显示了活化抗原。然而，它们在抗原呈递之外的作用尚未完全确定。在这项研究中，我们测试了选择性FDC消融对短期卵泡和GC功能的影响。FDC消融后2天内，原代卵泡失去了均匀性，成为T区周围无组织的细胞带。这些B细胞区保留了表达cxcl13的基质细胞，但经常表现出不适当的ER-TR7和CCL21表达。GC fdc消融导致GC消失。当使用Bcl2转基因防止B细胞死亡时，FDC消融导致脾GC B细胞分散。肠系膜淋巴结GCs耐药更强，但当鞘氨醇-1-磷酸受体-2也被移除时，GCs变得分散。这些实验表明，FDCs有助于维持原代卵泡作为B细胞专属的生态位，并确定了FDCs在GCs中保持细胞的关键作用。
Selective ablation of follicular dendritic cells in mice results in disorganization of primary follicles and dispersal of B cells out of splenic germinal centers. Follicular dendritic cells (FDCs) retain and display opsonized antigens in primary follicles and germinal centers (GCs). However, their roles beyond antigen presentation have been incompletely defined. In this study, we tested the impact of selective FDC ablation on short-term follicle and GC function. Within 2 d of FDC ablation, primary follicles lost their homogeneity and became disorganized bands of cells around T zones. These B cell areas retained CXCL13-expressing stromal cells but often exhibited inappropriate ER-TR7 and CCL21 expression. Ablation of GC FDCs led to the disappearance of GCs. When B cell death was prevented using a Bcl2 transgene, FDC ablation led to splenic GC B cell dispersal. Mesenteric lymph node GCs were more resistant but became dispersed when sphingosine-1-phosphate receptor-2 was also removed. These experiments indicate that FDCs help maintain primary follicles as a B cell exclusive niche and define a critical role for FDCs in cell retention within GCs.
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影响因子：
32.4
作者：
Kabashima, K;Banks, TA;Cyster, JG
通讯作者：
Cyster, JG
DOI：
10.1084/jem.20071019
发表时间：
2008-06-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kranich J;Krautler NJ;Heinen E;Polymenidou M;Bridel C;Schildknecht A;Huber C;Kosco-Vilbois MH;Zinkernagel R;Miele G;Aguzzi A
通讯作者：
Aguzzi A
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
64.8
作者：
Ansel, KM;Ngo, VN;Cyster, JG
通讯作者：
Cyster, JG
影响因子：
4.4
作者：
Cupedo, T;Lund, FE;Mebius, RE
通讯作者：
Mebius, RE