Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.
Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.
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数字基因表达标签分析调控小鼠精子发生早期的基因表达模式
DOI：
10.1371/journal.pone.0058680
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Jia M
中科院分区：
文献类型：
--
作者：
Zhang X;Hao L;Meng L;Liu M;Zhao L;Hu F;Ding C;Wang Y;He B;Pan Y;Fang W;Chen J;Hu S;Jia M
精原细胞和初级精母细胞中基因表达模式的详细表征对于理解正常精子发生过程中减数分裂前发生的过程至关重要。采用Solexa/Illumina数字基因表达系统（DGE），对小鼠B型精原细胞和初级精母细胞的全基因组表达谱进行了研究，系统分析了小鼠精子发生早期的发育过程。小鼠B型精原细胞和初级精母细胞之间的基因表达模式差异显着。功能分析显示，与连接组装，肌动蛋白细胞骨架和多能性的调节相关的基因表达差异最显着。通路分析表明，Wnt非经典信号通路在精原细胞发育过程中起着核心作用，并与肌动蛋白丝组装通路相互作用。本研究为进一步分析调控精子发生的基因表达模式和信号通路奠定了基础。
Detailed characterization of the gene expression patterns in spermatogonia and primary spermatocytes is critical to understand the processes which occur prior to meiosis during normal spermatogenesis. The genome-wide expression profiles of mouse type B spermatogonia and primary spermatocytes were investigated using the Solexa/Illumina digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental processes which occur during early spermatogenesis were systematically analyzed. Gene expression patterns vary significantly between mouse type B spermatogonia and primary spermatocytes. The functional analysis revealed that genes related to junction assembly, regulation of the actin cytoskeleton and pluripotency were most significantly differently expressed. Pathway analysis indicated that the Wnt non-canonical signaling pathway played a central role and interacted with the actin filament organization pathway during the development of spermatogonia. This study provides a foundation for further analysis of the gene expression patterns and signaling pathways which regulate the molecular mechanisms of early spermatogenesis.
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影响因子：
4
作者：
Hess, J;Angel, P;Schorpp-Kistner, M
通讯作者：
Schorpp-Kistner, M
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
4.1
作者：
Cawthorn WP;Bree AJ;Yao Y;Du B;Hemati N;Martinez-Santibañez G;MacDougald OA
通讯作者：
MacDougald OA
影响因子：
2.5
作者：
Cory, Aron T.;Boyer, Alexandre;Silversides, David W.
通讯作者：
Silversides, David W.
影响因子：
13.5
作者：
Gauglhofer, Christine;Sagmeister, Sandra;Grasl-Kraupp, Bettina
通讯作者：
Grasl-Kraupp, Bettina