Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells.
Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells.
复制标题
DOI：
10.1093/nar/gkn1025
复制
发表时间：
2009-03
影响因子：
14.9
通讯作者：
Fahrenkrug SC
中科院分区：
文献类型：
--
作者：
Clark KJ;Carlson DF;Leaver MJ;Foster LK;Fahrenkrug SC
DNA转座子的Tc 1/mariner家族广泛存在于真菌、植物和动物界，并被认为有助于其宿主基因组的进化。迄今为止，尚未在脊椎动物的天然基因组中鉴定出活性Tc 1转座子。我们证明，护照，从鱼（Pleuronectes platessa）分离的本地转座子，是活跃在各种脊椎动物细胞。在转座试验中，我们发现，护照转座子系统提高了40倍的稳定细胞转基因，有一个明显的偏好插入到基因中，并受到过度生产抑制像其他Tc 1元素。护照是第一个脊椎动物Tc 1元件描述为天然完整和功能活性，并考虑到其有限的系统发育分布，可能是同期活跃。因此，护照转座子系统补充了现有的遗传工具的脊椎动物基因组的操作，并可能提供一个独特的系统，研究脊椎动物基因组的渗透Tc 1元素。
The Tc1/mariner family of DNA transposons is widespread across fungal, plant and animal kingdoms, and thought to contribute to the evolution of their host genomes. To date, an active Tc1 transposon has not been identified within the native genome of a vertebrate. We demonstrate that Passport, a native transposon isolated from a fish (Pleuronectes platessa), is active in a variety of vertebrate cells. In transposition assays, we found that the Passport transposon system improved stable cellular transgenesis by 40-fold, has an apparent preference for insertion into genes, and is subject to overproduction inhibition like other Tc1 elements. Passport represents the first vertebrate Tc1 element described as both natively intact and functionally active, and given its restricted phylogenetic distribution, may be contemporaneously active. The Passport transposon system thus complements the available genetic tools for the manipulation of vertebrate genomes, and may provide a unique system for studying the infiltration of vertebrate genomes by Tc1 elements.
登录
查看更多内容
影响因子：
30.8
作者：
Garrick, D;Fiering, S;Whitelaw, E
通讯作者：
Whitelaw, E
影响因子：
3.5
作者：
Koga, Akihiko;Shimada, Atsuko;Hamaguchi, Satoshi
通讯作者：
Hamaguchi, Satoshi
影响因子：
5
作者：
Kong, Byung-Whi;Foster, Linda K.;Foster, Douglas N.
通讯作者：
Foster, Douglas N.
影响因子：
7.7
作者：
Klinakis, AG;Zagoraiou, L;Savakis, C
通讯作者：
Savakis, C
影响因子：
12.4
作者：
Geurts, AM;Yang, Y;Hackett, PB
通讯作者：
Hackett, PB