An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes.
An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes.
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DOI：
10.1186/s40813-018-0093-6
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发表时间：
2018
影响因子：
3.4
通讯作者：
Retana-Márquez S
中科院分区：
文献类型：
--
作者：
Casillas F;Betancourt M;Cuello C;Ducolomb Y;López A;Juárez-Rojas L;Retana-Márquez S
大多数研究是为了评估猪卵母细胞玻璃化冷冻后的恢复和发育，报道了在胚胎发育阶段或受精卵的冷冻保存效果较好，但在未成熟卵母细胞中则不是这样。为此，人们进行了许多研究来改进未成熟卵的玻璃化冷冻方案，以测试使用不同的冷冻保护剂浓度、冷却装置、孵化时间；但只有少数研究评估了哪种受精程序可以提高玻璃化冷冻卵母细胞的囊胚率。因此，本研究的目的是评估：1)注射前使用透明质酸(HA)或聚乙烯吡咯烷酮(PVP)的精子选择是否在提高受精率和囊胚形成率中起关键作用；2)采用体外受精(IVF)、卵胞浆内单精子注射(ICSI)和常规ICSI+透明质酸(HA)精子选择等不同的受精技术，对体外受精(IVF)、卵胞浆内单精子注射(ICSI)和常规ICSI+透明质酸(HA)单精子选择的猪未成熟卵母细胞进行体外受精(IVF)、胞浆内单精子注射(ICSI)和常规ICSI+透明质酸(HA)单精子选择，以提高胚胎发育能力和囊胚率。与对照组和暴露在PVP中的精子相比，HA-PICSI选择的精子显示出更高的活体/顶体反应状态。PVP暴露后的死亡/顶体反应率高于对照组和HA。在卵母细胞中，玻璃化冷冻后的卵母细胞存活率显著下降。此外，颗粒细胞培养的对照裸卵(DO-GC)与玻璃化冷冻卵母细胞的体外成熟率无明显差异。在受精参数方面，IVF在总受精率中所占比例高于ICSI和PICSI。然而，结果表明，与IVF和ICSI相比，PICSI受精提高了对照DO-GC和玻璃化冷冻卵母细胞的囊胚率。在玻璃化冷冻的GV卵母细胞中，为了获得高的囊胚形成率，建议在注射前用透明质酸而不是PVP来选择精子，因为这样可以获得高的存活率和顶体反应率。此外，与IVF和ICSI相比，PICSI受精是产生更高囊胚率的最好方法。
Most studies carried out to evaluate recovery and development after porcine oocyte vitrification, reported better rates when cryopreserved in embryonic development stages or zygotes, but not in immature oocytes. For this reason, many studies are performed to improve immature oocyte vitrification protocols testing the use of different cryoprotectant concentrations, cooling devices, incubation times; but only a few of them have evaluated which fertilization procedure enhances blastocyst rates in vitrified oocytes. Therefore, this study was aimed to evaluate: 1) if the sperm selection with hyaluronic acid (HA) or polyvinylpyrrolidone (PVP) before injection could play a key role in increasing fertilization and blastocyst formation and 2) the embryo developmental ability and blastocyst production of porcine immature oocytes retrieved after vitrification-warming and co-cultured with granulosa cells during IVM, using different fertilization techniques: in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and conventional ICSI with hyaluronic acid (HA) sperm selection, known as physiological intracytoplasmic sperm injection (PICSI) and. Sperm selected with HA-PICSI displayed a higher percentage of live/acrosome reacted status compared to those in control and exposed to PVP. Higher dead/acrosome reacted rates were obtained after PVP exposure compared to control and HA. In oocytes, viability significantly decreased after IVM in vitrified oocytes. Besides, IVM rates were not different between control denuded oocytes cultured with granulosa cells (DO-GC) and vitrified oocytes. Regarding fertilization parameters, IVF showed higher percentages of total fertilization rate than those obtained by ICSI and PICSI. However, results demonstrate that PICSI fertilization increased the blastocysts formation rate in control DO-GC and vitrified oocytes compared to IVF and ICSI. To achieve high blastocyst formation rates from vitrified GV oocytes, it is recommended that sperm should be selected with HA instead of PVP before injection since high viability and acrosome reaction rates were obtained. Also, PICSI fertilization was the best method to produce higher blastocyst rates compared to the IVF and ICSI procedures.
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影响因子：
2.7
作者：
Casillas, Fahiel;Teteltitla-Silvestre, Mario;Betancourt, Miguel
通讯作者：
Betancourt, Miguel
DOI：
10.1590/s0103-84782006000500024
发表时间：
2006-10-01
期刊：
Ciência Rural
影响因子：
--
作者：
Santos, Rodrigo Marques dos;Barreta, Marcos Henrique;Mezzalira, Alceu
通讯作者：
Mezzalira, Alceu
影响因子：
6.7
作者：
Bedaiwy, MA;Falcone, T;Agarwal, A
通讯作者：
Agarwal, A
影响因子：
1.2
作者：
Garcia-Mengual, E.;Silvestre, M. A.;Garcia-Rosello, E.
通讯作者：
Garcia-Rosello, E.
影响因子：
6.7
作者：
Godard NM;Pukazhenthi BS;Wildt DE;Comizzoli P
通讯作者：
Comizzoli P