Paracrine factors from cumulus-enclosed oocytes ensure the successful maturation and fertilization in vitro of denuded oocytes in the cat model.
Paracrine factors from cumulus-enclosed oocytes ensure the successful maturation and fertilization in vitro of denuded oocytes in the cat model.
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DOI：
10.1016/j.fertnstert.2008.05.069
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发表时间：
2009-05
影响因子：
6.7
通讯作者：
Comizzoli P
中科院分区：
文献类型：
--
作者：
Godard NM;Pukazhenthi BS;Wildt DE;Comizzoli P
利用cat模型更好地表征卵母细胞成熟和受精过程中卵丘与卵母细胞的相互作用。体外实验研究。史密森学会。家养短毛猫。将脱毛卵母细胞（DOs）和卵丘-卵母细胞复合物（COCs）进行体外成熟（IVM，加或不加FSH/LH，加或不加间隙连接干扰物1-庚醇，分开组或共培养）和体外受精（IVF，分开组或共培养）。比较不同体外条件下单独培养的DOs和与COCs共培养的DOs的核成熟度、原核形成、早期胚胎裂解动力学和囊胚形成/质量。在没有FSH/LH的情况下，去除积云细胞会阻止DOs的自发减数分裂恢复。使用FSH/LH，各组DOs进展到中期I期，但只有在与完整（未中断）COCs共培养时才完全进展到中期II期。单独培养的DOs组受精率较低，未形成囊胚。相比之下，在IVM和IVF期间，与完整COCs共培养的DOs恢复了受精率，约35%形成了囊胚。猫模型卵母细胞卵丘封闭产生的旁分泌因子将有助于研制成功体外培养DOs的合成培养基。
To better characterize the cumulus-oocyte interactions during oocyte maturation and fertilization using the cat model. Experimental in vitro study. Smithsonian Institution. Domestic Shorthair cats. Groups of denuded oocytes (DOs) and cumulus-oocyte complexes (COCs) were subjected to in vitro maturation (IVM; with or without FSH/LH, with or without the gap junction disruptor 1-heptanol, in separated groups or in co-culture) and inseminated in vitro (IVF; in separated groups or in co-culture). Nuclear maturation, pronuclear formation, kinetics of early embryo cleavage, and blastocyst formation/quality after different in vitro conditions were compared between DOs cultured separately and DOs co-cultured with COCs. Without FSH/LH, the removal of cumulus cells prevented spontaneous meiotic resumption in DOs. With FSH/LH, groups of DOs progressed to the metaphase I stage, but fully advanced to metaphase II only in co-culture with intact (non-disrupted) COCs. Groups of DOs cultured separately were poorly fertilized and exhibited no blastocyst formation. In contrast, DOs co-cultured with intact COCs during IVM and IVF recovered fertilizability and ~ 35% formed blastocysts. Paracrine factors produced by cumulus-enclosed oocytes in the cat model will help to develop synthetic media for successful in vitro culture of DOs.
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影响因子：
2.8
作者：
Fatehi, AN;Zeinstra, EC;Bevers, MM
通讯作者：
Bevers, MM
影响因子：
2.5
作者：
Ge, Li;Han, Dong;Tan, Jing-He
通讯作者：
Tan, Jing-He
影响因子：
3.6
作者：
Lequarre, AS;Marchandise, J;Donnay, I
通讯作者：
Donnay, I
DOI：
10.1186/1477-7827-1-14
发表时间：
2003-02-11
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Calder, Michele D;Caveney, Anita N;Watson, Andrew J
通讯作者：
Watson, Andrew J
影响因子：
2.5
作者：
Atef, A;François, P;Marc-André, S
通讯作者：
Marc-André, S