Wound healing and blastema formation in regenerating digit tips of adult mice.
Wound healing and blastema formation in regenerating digit tips of adult mice.
复制标题
成年小鼠的再生数字尖端中的伤口愈合和胚芽形成。
DOI：
10.1016/j.ydbio.2010.11.035
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发表时间：
2011-02-15
影响因子：
2.7
通讯作者：
Muneoka K
中科院分区：
文献类型：
--
作者：
Fernando WA;Leininger E;Simkin J;Li N;Malcom CA;Sathyamoorthi S;Han M;Muneoka K
小鼠终末指骨远端区域的截肢引起最初的伤口愈合反应，随后是芽基形成和指尖再生。到目前为止，大多数再生研究都集中在胚胎或新生儿模型，很少有研究探讨成人手指再生。在这里，我们报告的研究，包括形态学，免疫组织学和体积分析成人手指再生阶段。重新生成的数字与原始数字非常相似，但不是完美的替代品。指端的再分化通过膜内骨化发生，形成骨小梁网络，其取代了截肢的皮质骨。指胚由表达波形蛋白（一种通用间充质标记物）的增殖细胞组成，并且与成熟组织相比，含有较少的内皮细胞，表明血管分布减少。大多数表达干细胞标志物SCA-1的胚细胞也共表达内皮标志物CD 31，表明存在内皮祖细胞。伤口愈合期间的表皮闭合非常缓慢，其特征在于伤口表皮不能穿过截骨闭合。相反，伤口愈合阶段与破骨细胞反应相关，破骨细胞反应降解残端骨，使伤口表皮底切远端骨，导致新的再截肢反应。因此，再生过程从接近原始截肢平面的水平开始。
Amputation of the distal region of the terminal phalanx of mice causes an initial wound healing response followed by blastema formation and the regeneration of the digit tip. Thus far, most regeneration studies have focused in embryonic or neonatal models and few studies have examined adult digit regeneration. Here we report on studies that include morphological, immunohistological, and volumetric analyses of adult digit regeneration stages. The regenerated digit is grossly similar to the original, but is not a perfect replacement. Re-differentiation of the digit tip occurs by intramembranous ossification forming a trabecular bone network that replaces the amputated cortical bone. The digit blastema is comprised of proliferating cells that express vimentin, a general mesenchymal marker, and by comparison to mature tissues, contains fewer endothelial cells indicative of reduced vascularity. The majority of blastemal cells expressing the stem cell marker SCA-1, also co-express the endothelial marker CD31, suggesting the presence endothelial progenitor cells. Epidermal closure during wound healing is very slow and is characterized by a failure of the wound epidermis to close across amputated bone. Instead, the wound healing phase is associated with an osteoclast response that degrades the stump bone allowing the wound epidermis to undercut the distal bone resulting in a novel re-amputation response. Thus, the regeneration process initiates from a level that is proximal to the original plane of amputation.
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影响因子：
2.1
作者：
Muneoka, Ken;Allan, Christopher H.;Han, Manjong
通讯作者：
Han, Manjong
DOI：
10.1073/pnas.0905851106
发表时间：
2010-02-23
影响因子：
11.1
作者：
Agrawal, Vineet;Johnson, Scott A.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
2.9
作者：
Chadwick, Robert B.;Bu, Liming;Baylink, David J.
通讯作者：
Baylink, David J.
DOI：
10.1073/pnas.0907931107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Bely, Alexandra E.;Sikes, James M.
通讯作者：
Sikes, James M.
影响因子：
2.5
作者：
Kato, T;Miyazaki, K;Yoshizato, K
通讯作者：
Yoshizato, K