Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation
Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation
复制标题
细胞外基质组成决定对表皮生长因子受体激活的转录反应
DOI：
--
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发表时间：
2001
影响因子：
11.1
通讯作者：
J. Woodgett
中科院分区：
文献类型：
--
作者：
S. Yarwood;J. Woodgett
The transcriptional response to epidermal growth factor (EGF) was examined in a cultured cell model of adhesion. Gene expression was monitored in human embryonic kidney cells (HEK293) after attachment of cells to the extracellular matrix (ECM) proteins, laminin, and fibronectin, by using complementary DNA micorarrays printed with 1,718 individual human genes. Cluster analysis revealed that the influence of EGF on gene expression, either positive or negative, was largely independent of ECM composition. However, clusters of EGF-regulated genes were identified that were diagnostic of the type of ECM proteins to which cells were attached. In these clusters, attachment of cells to a laminin or fibronectin substrata specifically modified the direction of gene expression changes in response to EGF stimulation. For example, in HEK293 cells attached to fibronectin, EGF stimulated an increase in the expression of some genes; however, genes in the same group were nonresponsive or even suppressed in cells attached to laminin. Many of the genes regulated by EGF and ECM proteins in this manner are involved in ECM and cytoskeletal architecture, protein synthesis, and cell cycle control, indicating that cell responses to EGF stimulation can be dramatically affected by ECM composition.
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DOI：
10.1016/s0021-9258(17)37115-6
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Leco;R. Khokha;N. Pavloff;S. Hawkes;D. Edwards
通讯作者：
K. Leco;R. Khokha;N. Pavloff;S. Hawkes;D. Edwards
影响因子：
19
作者：
Lukashev, ME;Werb, Z
通讯作者：
Werb, Z
DOI：
10.1016/s0022-2143(97)90124-4
发表时间：
1997-11-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Grande, JP;Melder, DC;Zinsmeister, AR
通讯作者：
Zinsmeister, AR
EGF-R 依赖性角质形成细胞存活调节。
影响因子：
4
作者：
Rodeck,U;Jost,M;Kari,C;Shih,DT;Lavker,RM;Ewert,DL;Jensen,PJ
通讯作者：
Jensen,PJ
DOI：
10.1073/pnas.93.20.10614
发表时间：
1996-10-01
影响因子：
11.1
作者：
Schena, M;Shalon, D;Davis, RW
通讯作者：
Davis, RW