External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system
External hyphae of Rhizophagus irregularis DAOM 197198 are less sensitive to low pH than roots in arbuscular mycorrhizae: evidence from axenic culture system
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不规则根霉 DAOM 197198 的外部菌丝对低 pH 值的敏感性低于丛枝菌根的根：来自无菌培养系统的证据
DOI：
10.1111/1758-2229.12573
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发表时间：
2017-10
影响因子：
3.3
通讯作者：
Yao Qing
中科院分区：
文献类型：
--
作者：
Wang Ning;Feng Zengwei;Zhou Yang;Zhu Honghui;Yao Qing
植物根系和丛枝菌根真菌（AMF）的生长可以抑制低pH值，但是，它在很大程度上是未知的，这是更敏感的低pH值。本研究旨在比较外部菌丝（EH）和根的生理和分子响应低pH值的生长，发育和功能。我们建立了AM共生在一个两室系统（根室，RC;菌丝室，HC）使用AMF和转化毛状根，并将它们暴露于pH 6.5和/或pH 4.5。结果表明，pH 4.5显著降低了根细胞活力，而pH 6.5的EH减弱了这种影响。在RC或HC中，pH 4.5降低了生物量、P含量、定殖、根中ALP活性以及EH中ALP活性和多磷酸盐积累。在HC中，pH 4.5抑制EH中的GintPT表达，但在RC中不抑制。菌根响应LePT的表达显着减少了较低的殖民由于降低pH值在RC或HC，而非菌根响应LePT的表达不受影响。变异分配分析表明，EH对低pH的敏感性低于根系。低pH胁迫下根系与EH的相互作用值得进一步研究。
The growth of plant roots and arbuscular mycorrhizal fungi (AMF) can be inhibited by low pH; however, it is largely unknown which is more sensitive to low pH. This study aimed to compare the physiological and molecular responses of external hyphae (EH) and roots to low pH in terms of growth, development and functioning. We established AM symbiosis in a two-compartmented system (root compartment, RC; hyphal compartment, HC) using AMF and transformed hairy roots and exposed them to pH 6.5 and/or pH 4.5. The results showed that pH 4.5 significantly decreased root cell viability, while EH at pH 6.5 attenuated the effect. In either RC or HC, pH 4.5 reduced biomass, P content, colonization, ALP activity in roots, and ALP activity and polyphosphate accumulation in EH. GintPT expression in EH was inhibited by pH 4.5 in HC but not in RC. The expression of mycorrhiza-responsive LePTs was significantly reduced by the lower colonization due to decreased pH in either RC or HC, while the expression of non-mycorrhiza-responsive LePTs was not affected. Variation partitioning analysis indicated that EH was less sensitive to low pH than roots. The interactions between roots and EH under low pH stress merit further investigation.
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影响因子：
4.9
作者：
Joner, EJ;van Aarle, IM;Vosatka, M
通讯作者：
Vosatka, M
影响因子：
4.9
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VONUEXKULL, HR;MUTERT, E
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6.9
作者：
Koyama, H;Toda, T;Hara, T
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