Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success.
Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success.
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鉴定调节果蝇雄性生殖成功的微肽和多个次级细胞基因。
DOI：
10.1073/pnas.2001897118
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发表时间：
2021-04-13
影响因子：
11.1
通讯作者：
Maeda RK
中科院分区：
文献类型：
--
作者：
Immarigeon C;Frei Y;Delbare SYN;Gligorov D;Machado Almeida P;Grey J;Fabbro L;Nagoshi E;Billeter JC;Wolfner MF;Karch F;Maeda RK
在许多物种中，交配会引起雌性的生理变化，从而提高交配对的繁殖成功率。交配后反应（PMR）是由雄性精液蛋白与雌性生殖系统相互作用引起的。由于 PMR 在与人类健康和农业相关的许多昆虫物种中的重要性，我们检查了果蝇中产生大部分精液蛋白的一种重要腺体细胞类型的基因表达谱。有趣的是，在该物种 PMR 所必需的蛋白质中，我们发现由所谓的非编码转录物编码的微肽被产生并执行重要的生殖功能。此类微肽以前未被识别，但正在成为复杂生物过程中的重要参与者。即使在充分表征的基因组中，许多转录本也被认为是非编码 RNA (ncRNA)，仅仅是因为缺乏大的开放阅读框 (ORF)。然而，现在越来越清楚的是，许多小 ORF (smORF) 产生具有重要生物学功能的肽。在对果蝇产生精液的附属腺的一种重要细胞类型的核糖体结合转录组进行表征的过程中，我们检测到了一种以前被认为是非编码的 RNA，称为雄性特异性腹部 (msa)。值得注意的是，msa 嵌套在 Bithorax 复合体的 HOX 基因簇中，并且已知其内含子之一内含有 micro-RNA。我们发现这种 RNA 编码一种“微肽”（9 或 20 个氨基酸，MSAmiP），仅在雄性副腺的次级细胞中表达，似乎在细胞核中积累。重要的是，这种微肽功能的丧失会导致精子竞争缺陷。除了深入了解罕见细胞类型的生物学之外，这项工作还强调了小肽的重要性，小肽是一类分子，目前正在成为复杂生物过程中的重要参与者。
In many species, mating induces physiological changes in the female that increase the reproductive success of the mating pair. The postmating response (PMR) is caused by male seminal fluid proteins interacting with the female reproductive system. Because of the importance of the PMR in many insect species relevant to human health and agriculture, we examined the gene expression profile of one important cell type of the gland that produces most of the seminal fluid proteins in Drosophila. Interestingly, among proteins necessary for the PMR in this species, we discovered that a micropeptide encoded by a supposedly noncoding transcript is produced and carries out important reproductive functions. Such micropeptides were previously unrecognized but are emerging as important actors in complex biological processes. Even in well-characterized genomes, many transcripts are considered noncoding RNAs (ncRNAs) simply due to the absence of large open reading frames (ORFs). However, it is now becoming clear that many small ORFs (smORFs) produce peptides with important biological functions. In the process of characterizing the ribosome-bound transcriptome of an important cell type of the seminal fluid-producing accessory gland of Drosophila melanogaster, we detected an RNA, previously thought to be noncoding, called male-specific abdominal (msa). Notably, msa is nested in the HOX gene cluster of the Bithorax complex and is known to contain a micro-RNA within one of its introns. We find that this RNA encodes a “micropeptide” (9 or 20 amino acids, MSAmiP) that is expressed exclusively in the secondary cells of the male accessory gland, where it seems to accumulate in nuclei. Importantly, loss of function of this micropeptide causes defects in sperm competition. In addition to bringing insights into the biology of a rare cell type, this work underlines the importance of small peptides, a class of molecules that is now emerging as important actors in complex biological processes.
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DOI：
10.1083/jcb.201401072
发表时间：
2014-09-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Corrigan L;Redhai S;Leiblich A;Fan SJ;Perera SM;Patel R;Gandy C;Wainwright SM;Morris JF;Hamdy F;Goberdhan DC;Wilson C
通讯作者：
Wilson C
影响因子：
7
作者：
Hanada, Kousuke;Zhang, Xu;Shiu, Shin-Han
通讯作者：
Shiu, Shin-Han
影响因子：
4.5
作者：
Gummalla M;Maeda RK;Castro Alvarez JJ;Gyurkovics H;Singari S;Edwards KA;Karch F;Bender W
通讯作者：
Bender W
影响因子：
2.6
作者：
BERTRAM, MJ;AKERKAR, GA;WOLFNER, MF
通讯作者：
WOLFNER, MF
影响因子：
1.2
作者：
Bertin, Benjamin;Renaud, Yoan;Junion, Guillaume
通讯作者：
Junion, Guillaume