An in vitro approach to identifying specificity determinants of mutagenesis mediated by DNA misalignments.
An in vitro approach to identifying specificity determinants of mutagenesis mediated by DNA misalignments.
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一种鉴定由 DNA 错位介导的诱变特异性决定因素的体外方法。
DOI：
10.1016/0022-2836(91)80177-v
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发表时间：
1991
影响因子：
5.6
通讯作者：
L. S. Ripley
中科院分区：
文献类型：
--
作者：
C. Papanicolaou;L. S. Ripley
在体外，在DNA聚合过程中，新合成的（引物）链的错配导致缺失和/或复杂的移码突变。在体外，重复和准回文DNA序列的类似错配被预测为诱变的中间体。致突变性错配由新合成DNA链3′-OH端序列与模板或新合成DNA中序列之间的互补配对介导。当错配引物作为DNA聚合的底物时，产生突变序列。负责检测到的突变体序列的错位进行了比较，类似的错位，不涉及诱变，和所有错位的可能性进行了比较，在聚合过程中暂停的位置由大肠杆菌聚合酶I或其Klenow片段。这些比较揭示了体外错位特异性的三个特征。首先，由停顿产生的末端很可能是致突变失调的前兆。第二，从检测到的光谱中没有一些潜在的错配可以很好地解释为它们产生的突变序列的预测不可检测性。第三，与停顿和突变体可检测性不同的因素是导致E介导的错配诱变特异性差异的原因。coliDNA聚合酶I和Klenow聚合酶。
In vitro, misalignments of the newly synthesized (primer) strand during DNA polymerization lead to deletion and/or complex frameshift mutations.In vitro, similar misalignments of repeated and quasipalindromic DNA sequences are predicted to be intermediates of mutagenesis. The mutagenic misalignments are mediated by complementary pairing between the sequence at the 3′-OH end of the newly synthesized DNA strand and sequences in the template or in the newly synthesized DNA. Mutant sequences are produced when the misaligned primers act as substrates for DNA polymerization. The misalignments responsible for detected mutant sequences were compared to similar misalignments that were not implicated in mutagenesis, and all misalignment possibilities were compared to the position of pausing during polymerization byEscherichia colipolymerase I or its Klenow fragment. These comparisons revealed three characteristics ofin vitromisalignment specificity. First, the termini produced by pausing are likely to be precursors to mutagenic misalignments. Second, the absence of some potential misalignments from the detected spectrum is explained well by the predicted undetectability of the mutant sequences they produce. Third, factors distinct from pausing and mutant detectability are responsible for differences in the specificity of misalignment mutagenesis mediated byE. coliDNA polymerase I and Klenow polymerase duringin vitrosynthesis.
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移码诱变 DNA 中间体的聚合酶特异性差异。
影响因子：
5.6
作者：
Papanicolaou,C;Ripley,LS
通讯作者：
Ripley,LS
影响因子：
2.9
作者：
LADUCA, RJ;FAY, PJ;BAMBARA, RA
通讯作者：
BAMBARA, RA
影响因子：
3.3
作者：
Kazic,T;Berg,DE
通讯作者：
Berg,DE
体外 DNA 合成过程中 O6-甲基脱氧鸟苷单磷酸的掺入动力学。
影响因子：
2.9
作者：
Snow,ET;Foote,RS;Mitra,S
通讯作者：
Mitra,S
DOI：
10.1073/pnas.79.4.983
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KAGUNI, LS;CLAYTON, DA
通讯作者：
CLAYTON, DA