Anti-Photoaging Effects of Low Molecular-Weight Fucoidan on Ultraviolet B-Irradiated Mice.
Anti-Photoaging Effects of Low Molecular-Weight Fucoidan on Ultraviolet B-Irradiated Mice.
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DOI：
10.3390/md16080286
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发表时间：
2018-08-18
期刊：
影响因子：
5.4
通讯作者：
Oh TH
中科院分区：
文献类型：
--
作者：
Kim YI;Oh WS;Song PH;Yun S;Kwon YS;Lee YJ;Ku SK;Song CH;Oh TH
紫外线(UV)B辐射可诱导DNA损伤和产生活性氧(ROS)，从而通过炎症信号通路和细胞外基质重塑蛋白、胶原蛋白和基质金属蛋白酶(MMPs)的调节而导致皮肤光老化。鉴于低分子岩藻糖胶(LMF)具有潜在的抗氧化和抗炎作用，我们研究了LMF对UVB诱导的光老化的保护作用。分别以2.0、1.0和0.2 mg/cm2(分别为LMF2.0、LMF1.0和LMF0.2)外用杨梅素或LMF0.2，每日1次，共治疗15周。与蒸馏水处理的光老化模型(UVB对照)相比，处理区域的皱纹形成、炎症、氧化应激、基质金属蛋白酶的表达和细胞凋亡情况进行了比较。与UVB对照组相比，LMF治疗，特别是LMF2.0和LMF1.0，显著抑制了皱纹的形成、皮肤水肿和中性粒细胞聚集到光损伤的损伤中。在降低IL-1β释放的同时，增加IL-10的表达。LMF处理抑制了氧化胁迫(丙二醛和超氧阴离子)，增强了内源抗氧化剂(谷胱甘肽)。此外，LMF还降低了MMP1、9和13mRNA的表达。组织病理学分析表明，LMF的抗光老化作用是通过其抗氧化、抗细胞凋亡和抑制MMP9的作用实现的。这些都表明LMF可以被用作光老化的皮肤保护药物。
Ultraviolet (UV) B exposure induces DNA damage and production of reactive oxygen species (ROS), which causes skin photoaging through signaling pathways of inflammation and modulation of extracellular matrix remodeling proteins, collagens, and matrix metalloproteinase (MMP). As low molecular-weight fucoidan (LMF) has potential antioxidant and anti-inflammatory properties, we examined the protective effects of LMF against UVB-induced photoaging. A UVB-irradiated mouse model was topically treated with myricetin or LMF at 2.0, 1.0 and 0.2 mg/cm2 (LMF2.0, LMF1.0 and LMF0.2, respectively) once a day for 15 weeks. Wrinkle formation, inflammation, oxidative stress, MMP expression, and apoptosis in the treated regions were compared with those in a distilled water-treated photoaging model (UVB control). LMF treatments, particularly LMF2.0 and LMF1.0, significantly inhibited the wrinkle formation, skin edema, and neutrophil recruitment into the photo-damaged lesions, compared with those in the UVB control. While LMF decreased interleukin (IL)-1β release, it increased IL-10. The LMF treatment inhibited the oxidative stresses (malondialdehyde and superoxide anion) and enhanced endogenous antioxidants (glutathione). Additionally, LMF reduced the mRNA expression of MMP-1, 9, and 13. The histopathological analyses revealed the anti-photoaging effects of LMF exerted via its antioxidant, anti-apoptotic, and MMP-9-inhibiting effects. These suggest that LMF can be used as a skin-protective remedy for photoaging.
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DOI：
10.4161/derm.24539
发表时间：
2013-01-01
期刊：
Dermato-endocrinology
影响因子：
--
作者：
Krause R
通讯作者：
Krause R
影响因子：
2.7
作者：
Kang, Su-Jin;Choi, Beom-Rak;Lee, Young-Joon
通讯作者：
Lee, Young-Joon
影响因子：
7.5
作者：
Matou, S;Helley, D;Fischer, AM
通讯作者：
Fischer, AM
影响因子：
5.6
作者：
MCCAFFREY, TA;FALCONE, DJ;BORTH, W
通讯作者：
BORTH, W
岩藻依氨基生物活性的重要决定因素：对棕色海藻中含有岩藻糖硫化的多糖的结构功能关系和提取方法的批判性回顾。
影响因子：
5.4
作者：
Ale MT;Mikkelsen JD;Meyer AS
通讯作者：
Meyer AS