Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.
Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.
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DOI：
10.1016/j.cub.2012.03.058
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发表时间：
2012-05-22
期刊：
影响因子：
9.2
通讯作者：
Pelegri, Francisco
中科院分区：
文献类型：
--
作者：
Lindeman, Robin E.;Pelegri, Francisco
中心体在有丝分裂和细胞分裂过程中作为微管组织者发挥着重要作用。此外，它通过形成一个微管母核，使雌性原核向雄性原核迁移，从而促进受精后亲本单倍体基因组的结合。精子和原核之间的稳定联系在这种定向运动中是必不可少的。我们的研究揭示了斑马鱼基因无用循环（fue）在受精卵中是雄性原核-中心体附着和雌性原核迁移所必需的。我们发现fue编码一种新的，母体提供的长形式淋巴细胞限制性膜蛋白（lrmp），这是一种功能未知的脊椎动物特异性基因。母体的lrmp mRNA和蛋白都高度定位于受精卵，在核膜、中心体和纺锤体上有很大程度的重叠。截断的Lrmp- egfp融合蛋白在Lrmp的c端识别亚细胞靶向信号，然而内源性mRNA定位可能对确保该蛋白的严格空间表达很重要。在完全突变胚胎中，Lrmp蛋白和Lrmp RNA的定位都存在缺陷，这表明Lrmp基因产物的正确靶向依赖于Lrmp功能。Lrmp是一种保守的脊椎动物基因，其母体遗传产物在受精过程中对核-中心体附着和原核重组至关重要。lrmp产物的精确亚细胞定位也表明，在胚胎早期，它们的功能需要严格的时空调节。
The centrosome has a well-established role as a microtubule organizer during mitosis and cytokinesis. In addition, it facilitates the union of parental haploid genomes following fertilization by nucleating a microtubule aster along which the female pronucleus migrates towards the male pronucleus. Stable associations between the sperm aster and the pronuclei are essential during this directed movement. Our studies reveal that the zebrafish gene futile cycle (fue) is required in the zygote for male pronucleus-centrosome attachment and female pronuclear migration. We show that fue encodes a novel, maternally-provided long form of lymphoid-restricted membrane protein (lrmp), a vertebrate-specific gene of unknown function. Both maternal lrmp mRNA and protein are highly localized in the zygote, in a largely overlapping pattern at nuclear membranes, centrosomes, and spindles. Truncated Lrmp-EGFP fusion proteins identified subcellular targeting signals in the C-terminus of Lrmp, however endogenous mRNA localization is likely important to ensure strict spatial expression of the protein. Localization of both Lrmp protein and lrmp RNA is defective in fue mutant embryos, indicating that correct targeting of lrmp gene products is dependent on Lrmp function. Lrmp is a conserved vertebrate gene whose maternally-inherited products are essential for nucleus-centrosome attachment and pronuclear congression during fertilization. Precise subcellular localization of lrmp products also suggests a requirement for strict spatiotemporal regulation of their function in the early embryo.
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DOI：
10.1083/jcb.200702026
发表时间：
2007-09-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Salpingidou G;Smertenko A;Hausmanowa-Petrucewicz I;Hussey PJ;Hutchison CJ
通讯作者：
Hutchison CJ
影响因子：
4
作者：
Payne, C;Rawe, V;Schatten, G
通讯作者：
Schatten, G
影响因子：
4.7
作者：
Manenti, Giacomo;Galbiati, Federica;Dragani, Tommaso A.
通讯作者：
Dragani, Tommaso A.
影响因子：
4.5
作者：
Mathavan S;Lee SG;Mak A;Miller LD;Murthy KR;Govindarajan KR;Tong Y;Wu YL;Lam SH;Yang H;Ruan Y;Korzh V;Gong Z;Liu ET;Lufkin T
通讯作者：
Lufkin T
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF