A method combining a kit with the Bionic e-Eye for rapid on site detection of diarrhetic shellfish poisoning
A method combining a kit with the Bionic e-Eye for rapid on site detection of diarrhetic shellfish poisoning
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一种试剂盒与仿生电眼相结合的腹泻性贝类中毒现场快速检测方法
DOI：
10.1039/c8ay00676h
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发表时间：
2018-06
影响因子：
3.1
通讯作者：
Wang Ping
中科院分区：
文献类型：
--
作者：
Qiu Xianxin;Zhong Longjie;Gan Ying;Su Kaiqi;Hu Shihui;Wang Ping
冈田酸是一种广泛存在于贝类中的脂溶性贝类毒素。贝类毒素污染的现场快速检测是一个严重的问题。为此，建立了一种将试剂盒与仿生电子眼相结合的现场快速检测软冈田酸的新方法。优化后，试剂盒达到了非常低的检测限，0.19 μg L−1，线性检测范围为0.2-10 μg L−1。与甲藻毒素1的交叉反应率为23.6%。该试剂盒与石房蛤毒素、雌蛤毒素2和3、果胶毒素2-B等毒素有少量交叉反应。回收率在75.78- 102.56%之间，变异系数小于20%。在PBS和贝类背景中，在仿生电子眼和酶标仪之间进行相关性分析。仿生电子眼对真实的贝类样品的检测结果与高效液相色谱法的检测结果具有很好的一致性。执行检测所花费的总时间小于90分钟。该方法与仿生电子眼集成试剂盒非常适合现场快速检测软冈田酸。
Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
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影响因子：
2.9
作者：
G. Volpe;E. Cotroneo;D. Moscone;L. Croci;L. Cozzi;G. Ciccaglioni;G. Palleschi
通讯作者：
G. Volpe;E. Cotroneo;D. Moscone;L. Croci;L. Cozzi;G. Ciccaglioni;G. Palleschi
DOI：
10.1271/bbb1961.51.877
发表时间：
1987-03
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
Jong-Soo Lee;T. Yanagi;Ritsuko Kenma;T. Yasumoto
通讯作者：
Jong-Soo Lee;T. Yanagi;Ritsuko Kenma;T. Yasumoto
影响因子：
6.2
作者：
A. Tang;M. Pravda;G. Guilbault;S. Piletsky;A. Turner
通讯作者：
A. Tang;M. Pravda;G. Guilbault;S. Piletsky;A. Turner
影响因子：
8.4
作者：
Su, Kaiqi;Zou, Quchao;Wang, Ping
通讯作者：
Wang, Ping
影响因子：
6.2
作者：
Campas, Monica;Marty, Jean-Louis
通讯作者：
Marty, Jean-Louis