The phylogenetically-related pattern recognition receptors EFR and XA21 recruit similar immune signaling components in monocots and dicots.
The phylogenetically-related pattern recognition receptors EFR and XA21 recruit similar immune signaling components in monocots and dicots.
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DOI：
10.1371/journal.ppat.1004602
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发表时间：
2015-01
期刊：
影响因子：
6.7
通讯作者：
Zipfel C
中科院分区：
文献类型：
--
作者：
Holton N;Nekrasov V;Ronald PC;Zipfel C
在植物免疫过程中，表面定位的模式识别受体（PRRs）识别病原相关分子模式（PAMP）。PRRs在植物物种间的转移是一种很有前途的广谱抗病工程策略。因此，有很大的兴趣，在不同的植物物种中了解PRR介导的抗性的机制。两种充分表征的植物PRR是分别来自拟南芥（Arabidopsis thaliana）和水稻的富含亮氨酸重复序列受体激酶（LRR-RKs）EFR和XA 21。有趣的是，尽管EFR和XA 21在进化上相距遥远，但它们在遗传学上密切相关，并且都是包含许多潜在PRR的LRR-RKs亚家族XII的成员。在这里，我们比较了这些相关的PRR在单子叶-双子叶植物分类学划分中参与免疫信号传导的能力。使用拟南芥EFR和水稻XA 21之间的嵌合体，我们表明，激酶结构域的水稻XA 21是功能触发elf 18诱导的信号和定量免疫细菌假单胞菌pv.番茄（Pto）DC 3000和根癌农杆菌（Agrobacterium tumefaciens）在拟南芥中的表达。此外，EFR：XA 21嵌合体以配体依赖性方式与EFR复合物的已知组分动态关联。相反，EFR与拟南芥同源的水稻XA 21相互作用蛋白，这似乎是参与EFR介导的信号和免疫拟南芥。我们的工作表明，在单子叶植物和双子叶植物之间，作用于不同LRR-RK型PRR下游的免疫组分的整体功能保守性。虫害和疾病造成重大农业损失。植物通过质膜定位的免疫受体（称为模式识别受体或PRR）识别病原体衍生的分子，导致病原体抗性。近年来，PRRs在植物物种间的转移已成为提高作物抗病性的一种有前途的生物技术方法。PRR的成功转移表明免疫信号传导组分在植物物种中是保守的。在这项研究中，我们证明了来自单子叶植物水稻的PRR XA 21在双子叶植物拟南芥（Arabidopsis thaliana）中具有功能，并且它可以定量增强对细菌的抗性。此外，我们表明，水稻XA 21和拟南芥EFR，这是进化上遥远的，但在遗传上密切相关，招募类似的信号转导元件的功能，揭示了整个保护免疫途径在单子叶植物和双子叶植物。这些发现表明PRRs下游信号的进化保守性，并表明PRRs在不同植物家族之间，以及单子叶植物和双子叶植物之间的转移是可能的。
During plant immunity, surface-localized pattern recognition receptors (PRRs) recognize pathogen-associated molecular patterns (PAMPs). The transfer of PRRs between plant species is a promising strategy for engineering broad-spectrum disease resistance. Thus, there is a great interest in understanding the mechanisms of PRR-mediated resistance across different plant species. Two well-characterized plant PRRs are the leucine-rich repeat receptor kinases (LRR-RKs) EFR and XA21 from Arabidopsis thaliana (Arabidopsis) and rice, respectively. Interestingly, despite being evolutionary distant, EFR and XA21 are phylogenetically closely related and are both members of the sub-family XII of LRR-RKs that contains numerous potential PRRs. Here, we compared the ability of these related PRRs to engage immune signaling across the monocots-dicots taxonomic divide. Using chimera between Arabidopsis EFR and rice XA21, we show that the kinase domain of the rice XA21 is functional in triggering elf18-induced signaling and quantitative immunity to the bacteria Pseudomonas syringae pv. tomato (Pto) DC3000 and Agrobacterium tumefaciens in Arabidopsis. Furthermore, the EFR:XA21 chimera associates dynamically in a ligand-dependent manner with known components of the EFR complex. Conversely, EFR associates with Arabidopsis orthologues of rice XA21-interacting proteins, which appear to be involved in EFR-mediated signaling and immunity in Arabidopsis. Our work indicates the overall functional conservation of immune components acting downstream of distinct LRR-RK-type PRRs between monocots and dicots. Pests and diseases cause significant agricultural losses. Plants recognize pathogen-derived molecules via plasma membrane-localized immune receptors (called pattern recognition receptors or PRRs), resulting in pathogen resistance. In recent years, the transfer of PRRs across plant species has emerged as a promising biotechnological approach to improve crop disease resistance. Successful transfers of PRRs suggest that immune signaling components are conserved across plant species. In this study, we demonstrate that the PRR XA21 from the monocot plant rice is functional in the dicot plant Arabidopsis thaliana (Arabidopsis) and that it confers quantitatively enhanced resistance to bacteria. Furthermore, we show that the rice XA21 and the Arabidopsis EFR, which are evolutionary-distant but phylogenetically closely related, recruit similar signaling components for their function, revealing an overall conservation of immune pathways across monocots and dicots. These findings demonstrate evolutionary conservation of downstream signaling from PRRs and indicate that transfer of PRRs is possible between different plant families, but also between monocots and dicots.
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DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
7.2
作者：
Jiang, Yingnan;Chen, Xiuhua;Song, Wen-Yuan
通讯作者：
Song, Wen-Yuan
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
影响因子：
16
作者：
Kadota, Yasuhiro;Sklenar, Jan;Zipfel, Cyril
通讯作者：
Zipfel, Cyril