Periosteum Mimetic Coating on Structural Bone Allografts via Electrospray Deposition Enhances Repair and Reconstruction of Segmental Defects.
Periosteum Mimetic Coating on Structural Bone Allografts via Electrospray Deposition Enhances Repair and Reconstruction of Segmental Defects.
复制标题
DOI：
10.1021/acsbiomaterials.0c00421
复制
发表时间：
2020-11-09
影响因子：
5.8
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Zhuang Z;John JV;Liao H;Luo J;Rubery P;Mesfin A;Boda SK;Xie J;Zhang X
结构性同种异体骨移植仍然是修复和重建大面积骨缺损的常用策略之一。由于覆盖皮质骨外表面的骨膜的损失，同种异体骨的愈合和结合非常缓慢和有限。为了提高同种异体移植物的生物学性能，在此，我们报告了一种新的和简单的方法，通过聚合物介导的电喷雾沉积在结构性骨同种异体移植物表面上的骨膜模拟涂层的工程。这种方法使得同种异体移植物上的涂层具有精确控制的成分和厚度。此外，通过使用适当的可生物降解的聚合物或聚合物共混物，可以定制骨膜模拟涂层以实现所需的药物释放曲线。在鼠节段性股骨缺损模型中进行的有效性研究表明，由聚乳酸-羟基乙酸共聚物（PLGA）和骨形态发生蛋白-2（BMP-2）模拟肽组成的同种异体移植物涂层显著改善了同种异体移植物愈合，如纤维化组织形成减少、骨膜骨形成增加和骨整合增强所证明。本研究为促进同种异体骨愈合的工程化骨膜仿生涂层提供了技术平台。这项技术最终可能导致一个现成的和多功能的结构性骨同种异体移植物，用于大段骨缺损的高效修复和重建。该技术还可用于通过修改其表面来改善其他医疗植入物的性能。
Structural bone allograft transplantation remains one of the common strategies for repair and reconstruction of large bone defects. Due to the loss of periosteum that covers the outer surface of the cortical bone, the healing and incorporation of allograft is extremely slow and limited. To enhance the biological performance of allograft, herein, we report a novel and simple approach for engineering of a periosteum mimetic coating on the surface of structural bone allografts via polymer-mediated electrospray deposition. This approach enables the coating on allograft with precisely controlled composition and thickness. In addition, the periosteum mimetic coating can be tailored to achieve desired drug release profiles by making use of an appropriate biodegradable polymer or polymer blend. The efficacy study in a murine segmental femoral bone defect model demonstrates that the allograft coating composed of poly(lactic-co-glycolic acid) (PLGA) and bone morphogenetic protein-2 (BMP-2) mimicking peptide significantly improves allograft healing as evidenced by decreased fibrotic tissue formation, increased periosteal bone formation, and enhanced osseointegration. Taken together, this study provides a platform technology for engineering periosteum mimetic coating which can greatly promote bone allograft healing. This technology could eventually result in an off-the-shelf and multifunctional structural bone allograft for highly effective repair and reconstruction of large segmental bone defects. The technology can also be used to ameliorate the performance of other medical implants by modifying their surfaces.
登录
查看更多内容
使用可变的长度聚谷氨酸结构域，可调节从羟基磷灰石生物材料和同种异体移植骨中递送生物活性肽。
影响因子：
4.9
作者：
Culpepper, Bonnie K.;Webb, William M.;Bonvallet, Paul P.;Bellis, Susan L.
通讯作者：
Bellis, Susan L.
影响因子：
14
作者：
Culpepper, Bonnie K.;Bonvallet, Paul P.;Reddy, Michael S.;Ponnazhagan, Selvarangan;Bellis, Susan L.
通讯作者：
Bellis, Susan L.
DOI：
10.1007/978-1-62703-989-5_4
发表时间：
2014-01-01
期刊：
SKELETAL DEVELOPMENT AND REPAIR: METHODS AND PROTOCOLS
影响因子：
--
作者：
Dhillon, Robinder S.;Zhang, Longze;Xie, Chao
通讯作者：
Xie, Chao
影响因子：
14
作者：
Gogolewski, S;Pineda, L;B端sing, CM
通讯作者：
B端sing, CM
DOI：
10.1038/mt.2013.222
发表时间：
2014-02
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
通讯作者：
--