Streptococcus suis contains multiple phase-variable methyltransferases that show a discrete lineage distribution.
Streptococcus suis contains multiple phase-variable methyltransferases that show a discrete lineage distribution.
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DOI：
10.1093/nar/gky913
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发表时间：
2018-11-30
影响因子：
14.9
通讯作者：
Jennings MP
中科院分区：
文献类型：
--
作者：
Atack JM;Weinert LA;Tucker AW;Husna AU;Wileman TM;F Hadjirin N;Hoa NT;Parkhill J;Maskell DJ;Blackall PJ;Jennings MP
猪链球菌是猪的主要病原体，引起许多慢性和急性感染，并且也正在成为主要的人畜共患病病原体，特别是在东南亚。我们的研究的一个不同的人口S。suis表明该生物体含有I型和III型相可变甲基转移酶。在所有之前的例子中，甲基转移酶的相位变化导致基因组范围的甲基化差异，并导致多个基因的差异调节，该系统被称为相位可变调节子（phase-variable regulon）。我们假设I型和III型系统中的每个变体编码具有独特特异性的甲基转移酶，因此可以通过不同特异性之间的重组驱动的改组（I型）或通过简单序列重复的双相开关（III型）来控制不同的相变化。在这里，我们提出了从S.使用单分子实时甲基化组分析的SUIS。我们证明相位变化发生在I型和III型甲基转移酶，并显示甲基转移酶类型和存在，和人口进化枝之间的独特关联。此外，我们表明，相位可变的I型甲基转移酶可能是在一个高毒力的人畜共患病亚群的起源。
Streptococcus suis is a major pathogen of swine, responsible for a number of chronic and acute infections, and is also emerging as a major zoonotic pathogen, particularly in South-East Asia. Our study of a diverse population of S. suis shows that this organism contains both Type I and Type III phase-variable methyltransferases. In all previous examples, phase-variation of methyltransferases results in genome wide methylation differences, and results in differential regulation of multiple genes, a system known as the phasevarion (phase-variable regulon). We hypothesized that each variant in the Type I and Type III systems encoded a methyltransferase with a unique specificity, and could therefore control a distinct phasevarion, either by recombination-driven shuffling between different specificities (Type I) or by biphasic on-off switching via simple sequence repeats (Type III). Here, we present the identification of the target specificities for each Type III allelic variant from S. suis using single-molecule, real-time methylome analysis. We demonstrate phase-variation is occurring in both Type I and Type III methyltransferases, and show a distinct association between methyltransferase type and presence, and population clades. In addition, we show that the phase-variable Type I methyltransferase was likely acquired at the origin of a highly virulent zoonotic sub-population.
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影响因子：
11.8
作者：
Nghia, Ho Dang Trung;Hoa, Ngo Thi;Linh, Le Dieu;Campbell, James;Diep, To Song;Chau, Nguyen Van Vinh;Mai, Nguyen Thi Hoang;Hien, Tran Tinh;Spratt, Brian;Farrar, Jeremy;Schultsz, Constance
通讯作者：
Schultsz, Constance
影响因子：
3.2
作者：
Pericone, CD;Bae, D;Weiser, JN
通讯作者：
Weiser, JN
影响因子：
14.9
作者：
Loenen WA;Dryden DT;Raleigh EA;Wilson GG
通讯作者：
Wilson GG
影响因子：
14.9
作者：
Murray IA;Clark TA;Morgan RD;Boitano M;Anton BP;Luong K;Fomenkov A;Turner SW;Korlach J;Roberts RJ
通讯作者：
Roberts RJ
影响因子：
14.9
作者：
Bayliss CD;Bidmos FA;Anjum A;Manchev VT;Richards RL;Grossier JP;Wooldridge KG;Ketley JM;Barrow PA;Jones MA;Tretyakov MV
通讯作者：
Tretyakov MV