Type I restriction enzymes and their relatives.
Type I restriction enzymes and their relatives.
复制标题
DOI：
10.1093/nar/gkt847
复制
发表时间：
2014-01
影响因子：
14.9
通讯作者：
Wilson GG
中科院分区：
文献类型：
--
作者：
Loenen WA;Dryden DT;Raleigh EA;Wilson GG
I型限制性内切酶（REases）是具有单独的限制性（R）、甲基化（M）和DNA序列识别（S）亚基的大型五聚体蛋白。它们是第一个被发现和纯化的REases，但与非常有用的II型REases不同，它们尚未在分子生物学家的酶工具箱中找到一席之地。I型酶一直难以表征，但随着基因组分析揭示其基因，甲基化分析揭示其识别序列，这种情况正在改变。已经详细研究了几种第一类原因，关于它们的研究结果引起了更多的注意。在这篇文章中，我们讨论了生物化学，生物学和I型REases的调节方面，以及噬菌体和质粒进化以逃避它们的机制。I型REases具有通过结构域改组和重排改变序列特异性的显著能力。我们总结了导致这一发现的经典实验和观察，并讨论了这种能力如何依赖于酶及其S亚基的模块化组织。最后，我们描述了具有与I型酶共同特征的II型限制修饰系统的实例，重点是不同的IIG型酶。
Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II REases, they have yet to find a place in the enzymatic toolbox of molecular biologists. Type I enzymes have been difficult to characterize, but this is changing as genome analysis reveals their genes, and methylome analysis reveals their recognition sequences. Several Type I REases have been studied in detail and what has been learned about them invites greater attention. In this article, we discuss aspects of the biochemistry, biology and regulation of Type I REases, and of the mechanisms that bacteriophages and plasmids have evolved to evade them. Type I REases have a remarkable ability to change sequence specificity by domain shuffling and rearrangements. We summarize the classic experiments and observations that led to this discovery, and we discuss how this ability depends on the modular organizations of the enzymes and of their S subunits. Finally, we describe examples of Type II restriction–modification systems that have features in common with Type I enzymes, with emphasis on the varied Type IIG enzymes.
登录
查看更多内容
影响因子：
2.8
作者：
Bates, S;Roscoe, RA;Wilkins, BM
通讯作者：
Wilkins, BM
影响因子：
14.9
作者：
Kasarjian JK;Kodama Y;Iida M;Matsuda K;Ryu J
通讯作者：
Ryu J
影响因子：
14.9
作者：
Atanasiu, C;Su, TJ;Dryden, DTF
通讯作者：
Dryden, DTF
影响因子：
5.6
作者：
ARBER, W;DUSSOIX, D
通讯作者：
DUSSOIX, D
影响因子：
5.6
作者：
Bianco, PR;Hurley, EM
通讯作者：
Hurley, EM