Phase variable genes of Campylobacter jejuni exhibit high mutation rates and specific mutational patterns but mutability is not the major determinant of population structure during host colonization.
Phase variable genes of Campylobacter jejuni exhibit high mutation rates and specific mutational patterns but mutability is not the major determinant of population structure during host colonization.
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DOI：
10.1093/nar/gks246
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发表时间：
2012-07
影响因子：
14.9
通讯作者：
Tretyakov MV
中科院分区：
文献类型：
--
作者：
Bayliss CD;Bidmos FA;Anjum A;Manchev VT;Richards RL;Grossier JP;Wooldridge KG;Ketley JM;Barrow PA;Jones MA;Tretyakov MV
由于随机的、高频率的、可逆的突变，表面结构的相位变化发生在不同的细菌物种中。空肠弯曲菌的多个基因由于polyC/G片段的突变而经历时相可变基因表达。在C.空肠基因组。使用染色体定位的报告构建体测量这些束的转换率，并且观察到cj 1139（G8）和cj 0031（G9）的高转换率。从G8到G11的cj 1139道的改变增加了10倍的突变性，并改变了突变模式，从主要插入，以主要缺失。利用多重PCR技术，检测到了一些时相可变基因在传代过程中的“开/关”状态的主要变化。鸡的空肠利用观察到的开关率在一个随机的，理论模型的相位变化表现出突变性和遗传多样性之间的联系，但不能复制观察到的人口多样性。我们建议，模态重复数已经在C。jejuni基因组的遗传多样性是由于与这些polyC/G重复序列的突变模式相关的分子驱动因素，而不是通过选择特定的转换速率，并且除了突变漂移之外的因素是这种细菌病原体在宿主定殖期间产生遗传多样性的原因。
Phase variation of surface structures occurs in diverse bacterial species due to stochastic, high frequency, reversible mutations. Multiple genes of Campylobacter jejuni are subject to phase variable gene expression due to mutations in polyC/G tracts. A modal length of nine repeats was detected for polyC/G tracts within C. jejuni genomes. Switching rates for these tracts were measured using chromosomally-located reporter constructs and high rates were observed for cj1139 (G8) and cj0031 (G9). Alteration of the cj1139 tract from G8 to G11 increased mutability 10-fold and changed the mutational pattern from predominantly insertions to mainly deletions. Using a multiplex PCR, major changes were detected in ‘on/off’ status for some phase variable genes during passage of C. jejuni in chickens. Utilization of observed switching rates in a stochastic, theoretical model of phase variation demonstrated links between mutability and genetic diversity but could not replicate observed population diversity. We propose that modal repeat numbers have evolved in C. jejuni genomes due to molecular drivers associated with the mutational patterns of these polyC/G repeats, rather than by selection for particular switching rates, and that factors other than mutational drift are responsible for generating genetic diversity during host colonization by this bacterial pathogen.
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应急基因座中的站立遗传变异驱动了弯曲杆菌的空肠对新宿主的快速适应。
影响因子：
3.7
作者：
Jerome JP;Bell JA;Plovanich-Jones AE;Barrick JE;Brown CT;Mansfield LS
通讯作者：
Mansfield LS
DOI：
10.1038/nrmicro2145
发表时间：
2009-07
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.6
作者：
Karlyshev, AV;Champion, OL;Szymanski, CM
通讯作者：
Szymanski, CM
影响因子：
10.7
作者：
Hardwick, Robert J.;Tretyakov, Michael V.;Dubrova, Yuri E.
通讯作者：
Dubrova, Yuri E.
影响因子：
3.6
作者：
Richardson, AR;Stojiljkovic, I
通讯作者：
Stojiljkovic, I