Use of advanced recombinant lines to study the impact and potential of mutations affecting starch synthesis in barley.
Use of advanced recombinant lines to study the impact and potential of mutations affecting starch synthesis in barley.
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DOI：
10.1016/j.jcs.2013.12.012
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发表时间：
2014-03
影响因子：
3.8
通讯作者：
Smith, Alison M.
中科院分区：
文献类型：
--
作者：
Howard, Thomas P.;Fahy, Brendan;Leigh, Fiona;Howell, Phil;Powell, Wayne;Greenland, Andy;Trafford, Kay;Smith, Alison M.
研究了不同背景的4个淀粉合成突变对大麦淀粉和籽粒性状的影响。将lys5f （adp葡萄糖转运蛋白）、wax（颗粒结合淀粉合成酶）、isa1（脱支酶同淀粉酶1）和sex6（淀粉合成酶IIa）突变基因导入NFC Tipple中，得到具有不同供体亲本基因组贡献的突变型和野生型BC2F4家族。通过比较供体父母、BC2F4家族和NFC Tipple之间的淀粉和谷物特性，可以将突变的影响与遗传背景效应区分开来。无论遗传背景如何，蜡和性6突变对淀粉特性都有显著影响。性6突变使籽粒重和淀粉含量降低，而蜡突变则不能。lys5突变导致籽粒重和淀粉含量低，但β-葡聚糖含量异常高。isa1突变促进可溶性α-葡聚糖（植物糖原）的合成。与供体亲本相比，其掺入提高了籽粒重和总α-葡聚糖含量，但降低了植物糖原与淀粉的比值。这项研究表明，突变渗入到一个共同的商业背景中，提供了从供体亲本中无法获得的新见解。从不同背景中引入4个大麦淀粉合成突变为一个优良品种。无论遗传背景如何，蜡和性6突变对淀粉特性都有显著影响。Sex6和lys5突变降低了籽粒重，但lys5突变导致β-葡聚糖含量很高。渗入降低了isa1突变体的植物糖原（可溶性α-葡聚糖）与淀粉的比例。
The effects on barley starch and grain properties of four starch synthesis mutations were studied during the introgression of the mutations from diverse backgrounds into an elite variety. The lys5f (ADPglucose transporter), wax (granule-bound starch synthase), isa1 (debranching enzyme isoamylase 1) and sex6 (starch synthase IIa) mutations were introgressed into NFC Tipple to give mutant and wild-type BC2F4 families with different genomic contributions of the donor parent. Comparison of starch and grain properties between the donor parents, the BC2F4 families and NFC Tipple allowed the effects of the mutations to be distinguished from genetic background effects. The wax and sex6 mutations had marked effects on starch properties regardless of genetic background. The sex6 mutation conditioned low grain weight and starch content, but the wax mutation did not. The lys5 mutation conditioned low grain weight and starch content, but exceptionally high β-glucan contents. The isa1 mutation promotes synthesis of soluble α-glucan (phytoglycogen). Its introgression into NFC Tipple increased grain weight and total α-glucan content relative to the donor parent, but reduced the ratio of phytoglycogen to starch. This study shows that introgression of mutations into a common, commercial background provides new insights that could not be gained from the donor parent. Four barley starch synthesis mutations were introgressed from diverse backgrounds into an elite variety. wax and sex6 mutations had marked effects on starch properties regardless of genetic background. sex6 and lys5 mutations reduced grain weight, but the lys5 mutation led to very high β-glucan contents. Introgression reduced the phytoglycogen (soluble α-glucan) to starch ratio in isa1 mutants.
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影响因子：
7.4
作者：
Patron, NJ;Smith, AM;Denyer, K
通讯作者：
Denyer, K
影响因子：
3.8
作者：
Munck, L;Moller, B;Sondergaard, I
通讯作者：
Sondergaard, I
影响因子：
11.2
作者：
Srichuwong, Sathaporn;Isono, Naoto;Hisamatsu, Makoto
通讯作者：
Hisamatsu, Makoto
影响因子：
7.2
作者：
Burton, RA;Jenner, H;Denyer, K
通讯作者：
Denyer, K
影响因子：
14.8
作者：
Smith, Alison M.;Zeeman, Samuel C.
通讯作者：
Zeeman, Samuel C.