Immunohistochemical localization of AMPA-type glutamate receptor subunits in the striatum of rhesus monkey.
Immunohistochemical localization of AMPA-type glutamate receptor subunits in the striatum of rhesus monkey.
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DOI：
10.1016/j.brainres.2010.05.003
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发表时间：
2010-07-16
期刊：
影响因子：
2.9
通讯作者：
Reiner, Anton J.
中科院分区：
文献类型：
--
作者：
Deng, Yun-Ping;Shelby, Evan;Reiner, Anton J.
皮质纹状体和丘脑纹状体投射利用谷氨酸作为神经递质。它们对纹状体的影响部分是通过离子型AMPA型谷氨酸受体介导的，这种受体是由GluR1-4亚基组成的异构体。虽然AMPA亚基在啮齿动物基底节的细胞定位已有很好的研究，但在灵长类动物基底节中的细胞定位尚不清楚。因此，我们对GluR1-4在恒河猴基底节的分布进行了免疫组织化学研究，并对每种主要的神经元类型进行了表征。在纹状体，约65%的纹状体神经元表达GluR1，75%-79%的纹状体神经元表达GluR2或GluR2/3，仅2.5%的纹状体神经元表达GluR4。所有较大的胆碱能中间神经元(平均直径26.1μm)的神经元均呈中等强度的GluR1标记，而少数胆碱能中间神经元(平均直径为13.8μm)的所有神经元均高度富含GluR1。此外，在投射神经元的大小范围内(平均直径11.6μm)，略多于一半的神经元免疫标记有GluR1，其中约三分之一非常丰富。大约一半的胆碱能中间神经元被GluR2免疫标记，其余被GluR2免疫标记的神经元在大小和形状上与投射神经元一致(GluR2直径=10.7μm)，表明绝大多数纹状体投射神经元具有免疫可降解的GluR2。GluR2/3免疫标记也观察到了类似的结果。GluR4免疫标记的神经元大小相当于胆碱能中间神经元大小的一半，似乎所有位于小白蛋白能中间神经元大小范围内的神经元都有GluR4。这些结果表明，恒河猴纹状体投射神经元的AMPA受体亚单位组合与啮齿动物的相应神经元类型相似，因此它们对谷氨酸的AMPA反应可能与啮齿动物相似。
Corticostriatal and thalamostriatal projections utilize glutamate as their neurotransmitter. Their influence on striatum is mediated, in part, by ionotropic AMPA-type glutamate receptors, which are heteromers composed of GluR1-4 subunits. While the cellular localization of AMPA-type subunits in the basal ganglia has been well characterized in rodents, the cellular localization of AMPA subunits in primate basal ganglia is not. We thus carried out immunohistochemical studies of GluR1-4 distribution in rhesus monkey basal ganglia in conjunction with characterization of each major neuron type. In striatum, about 65% of striatal neurons immunolabeled for GluR1, 75%-79% immunolabeled for GluR2 or GluR2/3, and only 2.5% possessed GluR4. All neurons the large size of cholinergic interneurons (mean diameter 26.1μm) were moderately labeled for GluR1, while all neurons in the size range of parvalbuminergic interneurons (mean diameter 13.8μm) were intensely rich in GluR1. Additionally, somewhat more than half of neurons in the size range of projection neurons (mean diameter 11.6μm) immunolabeled for GluR1, and about one third of these were very rich in GluR1. About half of neurons the size of cholinergic interneurons were immunolabeled for GluR2, and the remainder of the neurons that were immunolabeled for GluR2 coincided with projection neurons in size and shape (GluR2 diameter=10.7μm), indicating that the vast majority of striatal projection neurons possess immunodectible GluR2. Similar results were observed with GluR2/3 immunolabeling. Half of the neurons the size of cholinergic interneurons immunolabeled for GluR4 and seemingly all neurons in the size range of parvalbuminergic interneurons possessed GluR4. These results indicate that AMPA receptor subunit combinations for striatal projection neurons in rhesus monkey are similar to those for the corresponding neuron types in rodents, and thus their AMPA responses to glutamate likely to be similar to those demonstrated in rodents.
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