Generation of conditional alleles for Foxc1 and Foxc2 in mice.
Generation of conditional alleles for Foxc1 and Foxc2 in mice.
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DOI：
10.1002/dvg.22036
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发表时间：
2012-10
期刊：
影响因子：
1.5
通讯作者：
Kume, Tsutomu
中科院分区：
文献类型：
--
作者：
Sasman, Amy;Nassano-Miller, Carey;Shim, Kyoo Seok;Koo, Hyun Young;Liu, Ting;Schultz, Kathryn M.;Millay, Meredith;Nanano, Atsushi;Kang, Myengmo;Suzuki, Takashi;Kume, Tsutomu
Forkhead box转录因子Foxc1和FOXC2对眼睛、心血管网络和其他生理系统的发育至关重要，但它们特定的细胞类型和发育后功能尚不清楚，部分原因是这两个因子的常规(即整个生物体)纯合子零突变会导致围产儿死亡。在这里，我们描述了通过Cre介导的重组诱导产生条件零Foxc1flx和Foxc2flx突变的小鼠。未重组等位基因纯合的小鼠是存活和可生育的，表明条件等位基因保留了它们的野生型功能。Foxc1或Foxc2 Flox小鼠与重组等位基因纯合的Cre-deleter小鼠杂交的胚胎(即Foxc1Δ/Δ或FoxC2Δ/Δ胚胎)缺乏相应基因的表达，并显示出与传统纯合子突变胚胎相同的发育缺陷。我们希望这些条件突变能够表征Foxc1和FOXC2在发育、疾病和成年动物中的细胞类型特定功能。
The Forkhead box transcription factors Foxc1 and Foxc2 are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell type–specific and post-developmental functions are unknown, in part because conventional (i.e., whole-organism) homozygous-null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional-null Foxc1flox and Foxc2flox mutations that are induced via Cre-mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild-type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre-deleter mice that are homozygous for the recombined allele (i.e., Foxc1Δ/Δ or Foxc2Δ/Δ embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell-type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals.
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影响因子：
11.8
作者：
Gitler, AD;Lu, MM;Epstein, JA
通讯作者：
Epstein, JA
DOI：
10.1016/j.bbrc.2007.12.183
发表时间：
2008-03-14
影响因子：
3.1
作者：
Hayashi, Hisaki;Kume, Tsutomu
通讯作者：
Kume, Tsutomu
影响因子：
30.8
作者：
Nishimura, DY;Swiderski, RE;Sheffield, VC
通讯作者：
Sheffield, VC
影响因子：
2.6
作者：
Hiemisch, H;Monaghan, AP;Kaestner, KH
通讯作者：
Kaestner, KH
DOI：
10.1073/pnas.0703900104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Mani, Sendurai A.;Yang, Jing;Weinberg, Robert A.
通讯作者：
Weinberg, Robert A.