Profiling antibody responses to infections by Chlamydia abortus enables identification of potential virulence factors and candidates for serodiagnosis.
Profiling antibody responses to infections by Chlamydia abortus enables identification of potential virulence factors and candidates for serodiagnosis.
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DOI：
10.1371/journal.pone.0080310
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Essig A
中科院分区：
文献类型：
--
作者：
Forsbach-Birk V;Foddis C;Simnacher U;Wilkat M;Longbottom D;Walder G;Benesch C;Ganter M;Sachse K;Essig A
由于感染专性细胞内病原体流产衣原体 (C.) 导致的母羊地方性动物流产 (EAE) 是一种重要的人畜共患疾病，给全世界农业带来了巨大的经济损失。该病原体可传播给人类，并可能导致孕妇严重感染。由于缺乏可靠的诊断工具，有关流行病学、临床病程和人类传播的知识受到阻碍。在受感染的动物和人类中表达的免疫反应蛋白可以作为诊断标记蛋白的新候选者并代表假定的毒力因子。为了拓宽免疫原性流产弯曲杆菌蛋白的谱系，我们应用了 2D 免疫印迹分析和使用人和动物血清筛选表达文库。我们已经鉴定了 48 种代表潜在诊断标记物和推定毒力因子的免疫反应蛋白，例如 CAB080（“巨噬细胞感染增强剂”MIP 的同源物）、CAB167（“易位肌动蛋白募集蛋白”TARP 的同源物）、CAB712（“衣原体蛋白酶样活性因子”CPAF 的同源物）、CAB776（“衣原体蛋白酶样活性因子”CPAF 的同源物）、CAB776（“易位肌动蛋白募集蛋白”TARP 的同源物） “多态性膜蛋白 D”(PmpD) 和“假设蛋白”CAB063、CAB408 和 CAB821，预计为 III 型分泌。我们选择了两个假定的毒力因子进行进一步表征，即 CAB080 (cMIP) 和 CAB063，并研究了它们在转录本和蛋白质水平上的表达谱。对这两种蛋白质在整个发育周期中的亚细胞定位的分析表明，CAB063 是第一个被证明易位至宿主细胞核的流产 C. abortus 蛋白质。
Enzootic abortion of ewes (EAE) due to infection with the obligate intracellular pathogen Chlamydia (C.) abortus is an important zoonosis leading to considerable economic loss to agriculture worldwide. The pathogen can be transmitted to humans and may lead to serious infection in pregnant women. Knowledge about epidemiology, clinical course and transmission to humans is hampered by the lack of reliable diagnostic tools. Immunoreactive proteins, which are expressed in infected animals and humans, may serve as novel candidates for diagnostic marker proteins and represent putative virulence factors. In order to broaden the spectrum of immunogenic C. abortus proteins we applied 2D immunoblot analysis and screening of an expression library using human and animal sera. We have identified 48 immunoreactive proteins representing potential diagnostic markers and also putative virulence factors, such as CAB080 (homologue of the “macrophage infectivity potentiator”, MIP), CAB167 (homologue of the “translocated actin recruitment protein”, TARP), CAB712 (homologue of the “chlamydial protease-like activity factor”, CPAF), CAB776 (homologue of the “Polymorphic membrane protein D”, PmpD), and the “hypothetical proteins” CAB063, CAB408 and CAB821, which are predicted to be type III secreted. We selected two putative virulence factors for further characterization, i.e. CAB080 (cMIP) and CAB063, and studied their expression profiles at transcript and protein levels. Analysis of the subcellular localization of both proteins throughout the developmental cycle revealed CAB063 being the first C. abortus protein shown to be translocated to the host cell nucleus.
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影响因子：
3.7
作者：
Kiselev, Andrey O.;Skinner, Megan C.;Lampe, Mary F.
通讯作者：
Lampe, Mary F.
影响因子：
6.7
作者：
Arnold R;Brandmaier S;Kleine F;Tischler P;Heinz E;Behrens S;Niinikoski A;Mewes HW;Horn M;Rattei T
通讯作者：
Rattei T
影响因子：
--
作者：
Marques, P. X.;Souda, Puneet;Nally, J. E.
通讯作者：
Nally, J. E.
影响因子：
7
作者：
Thomson, NR;Yeats, C;Longbottom, D
通讯作者：
Longbottom, D
影响因子：
3.1
作者：
CALDWELL, HD;KROMHOUT, J;SCHACHTER, J
通讯作者：
SCHACHTER, J