Differentiation of the O and P cell lines in the embryo of the leech. II. Genealogical relationship of descendant pattern elements in alternative developmental pathways.
Differentiation of the O and P cell lines in the embryo of the leech. II. Genealogical relationship of descendant pattern elements in alternative developmental pathways.
复制标题
水蛭胚胎中 O 和 P 细胞系的分化。
DOI：
10.1016/0012-1606(87)90431-3
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发表时间：
1987
影响因子：
2.7
通讯作者：
Shankland,M
中科院分区：
文献类型：
--
作者：
Shankland,M
原始细胞是在水蛭胚带的外胚层细胞层中发现的一组胚胎前体细胞。每个p胚细胞通常经历相同的不变序列的细胞分裂，并产生一个精确定义的一组独特的可识别的神经元和表皮的后代在成熟的水蛭。在本文中，各种p胚细胞后代注射荧光谱系示踪剂，以表征其后代克隆的细胞组成，结果表明，有一个定型的分离后代细胞的命运，通过前三个p胚细胞分裂。以前的工作已经表明，神经元和表皮的专业化，通常下降从p母细胞将出现从不同的受体-o母细胞-在响应消融相邻的P细胞系，如果o母细胞是在一定的分化阶段时，消融进行它将产生正常的P后代的一个子集。与目前的研究结果比较表明，在这些条件下，o胚细胞克隆不是简单地重演正常p胚细胞谱系的分支，而是表现出一种替代谱系，其中P后代表现出异常的系谱关系。因此，即使正常水蛭发展包括一个几乎不变的细胞谱系，谱系关系是开放的相当大的重组实验条件下。
The p blast cells are a group of embryonic precursors found in the ectodermal cell layer of the leech germinal band. Each p blast cell normally undergoes the same invariant sequence of cell divisions and gives rise to a precisely defined set of uniquely identifiable neuronal and epidermal descendants in the mature leech. In the present paper, various of the p blast cell progeny were injected with a fluorescent lineage tracer in order to characterize the cellular composition of their descendant clones, and the results show that there is a stereotyped segregation of descendant cell fates through the first three p blast cell divisions. Previous work has shown that neurons and epidermal specializations which normally descend from the p blast cell will arise from a different precursor—the o blast cell—in response to ablation of the neighboring P cell line and that if the o blast cell is at a certain stage of differentiation when the ablation is performed it will produce only a subset of the normal P descendants. Comparison with the present findings indicates that under those conditions the o blast cell clone is not simply recapitulating a branch of the normal p blast cell lineage, but rather manifests an alternative lineage in which P descendants exhibit an abnormal genealogical relationship. Thus, even though normal leech development comprises a nearly invariant cell lineage, lineage relationships are open to considerable reorganization under experimental conditions.
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影响因子：
2.7
作者：
Weisblat,DA;Blair,SS
通讯作者：
Blair,SS
影响因子：
2.7
作者：
Saul L. Zackson
通讯作者：
Saul L. Zackson
在水蛭胚胎中 O 和 P 细胞系的位置指定过程中，母细胞命运的逐步承诺。
影响因子：
2.7
作者：
Shankland,M;Weisblat,DA
通讯作者：
Weisblat,DA
影响因子：
2.7
作者：
DOE, CQ;GOODMAN, CS
通讯作者：
GOODMAN, CS
Rohon-Beard 神经元起源于 16 细胞青蛙胚胎的卵裂球
影响因子：
5.3
作者：
M. Jacobson
通讯作者：
M. Jacobson