Identification and Functional Analysis of lncRNA by CRISPR/Cas9 During the Cotton Response to Sap-Sucking Insect Infestation.
Identification and Functional Analysis of lncRNA by CRISPR/Cas9 During the Cotton Response to Sap-Sucking Insect Infestation.
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DOI：
10.3389/fpls.2022.784511
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发表时间：
2022
影响因子：
5.6
通讯作者：
Yuan D
中科院分区：
文献类型：
--
作者：
Zhang J;Li J;Saeed S;Batchelor WD;Alariqi M;Meng Q;Zhu F;Zou J;Xu Z;Si H;Wang Q;Zhang X;Zhu H;Jin S;Yuan D
植食性害虫对棉花生产造成严重危害。长链非编码RNA（longnoncodingRNAs，lncRNA）在棉花的各种发育过程和逆境应答中起着重要的调控作用，但其在棉花吸汁害虫侵染过程中的作用尚不清楚。比较了不同时间点（0、4、12、24和48 h）抗虫棉（HR）和感病棉（ZS）的转录组谱。从RNA-seq数据中鉴定了总共6，651个lncRNA转录物和606个差异表达的lncRNA。共表达网络表明，lncA 07和lncD 09是潜在的枢纽基因，在棉花防御蚜虫侵染中发挥调控作用。此外，lncD 09和lncA 07的CRISPR/Cas9敲除突变体显示出茉莉酸（JA）含量的降低，这可能导致对昆虫侵染的易感性增加。野生型和lncRNA敲除植物之间的差异表达基因在调节发育和对刺激的抗性中富集。此外，一些候选基因如Ghir_A01G022270、Ghir_D04G014430和Ghir_A01G022270参与JA介导的信号传导途径的调节。这一结果为lncRNA在棉花抗虫防御系统中的作用提供了新的见解。
Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection in cotton is largely unknown. In this study, the transcriptome profiles between resistant (HR) and susceptible (ZS) cotton cultivars under whitefly infestation at different time points (0, 4, 12, 24, and 48 h) were compared. A total of 6,651 lncRNAs transcript and 606 differentially expressed lncRNAs were identified from the RNA-seq data. A co-expression network indicated that lncA07 and lncD09 were potential hub genes that play a regulatory role in cotton defense against aphid infestation. Furthermore, CRISPR/Cas9 knock-out mutant of lncD09 and lncA07 showed a decrease of jasmonic acid (JA) content, which potentially lead to increased susceptibility toward insect infestation. Differentially expressed genes between wild type and lncRNA knock-out plants are enriched in modulating development and resistance to stimulus. Additionally, some candidate genes such as Ghir_A01G022270, Ghir_D04G014430, and Ghir_A01G022270 are involved in the regulation of the JA-mediated signaling pathway. This result provides a novel insight of the lncRNA role in the cotton defense system against pests.
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