Plasmid-cured Chlamydia caviae activates TLR2-dependent signaling and retains virulence in the guinea pig model of genital tract infection.
Plasmid-cured Chlamydia caviae activates TLR2-dependent signaling and retains virulence in the guinea pig model of genital tract infection.
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DOI：
10.1371/journal.pone.0030747
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
O'Connell CM
中科院分区：
文献类型：
--
作者：
Frazer LC;Darville T;Chandra-Kuntal K;Andrews CW Jr;Zurenski M;Mintus M;AbdelRahman YM;Belland RJ;Ingalls RR;O'Connell CM
从C. trachomatis和C.鼠伤寒沙门氏菌是多效性的，导致通过TLR 2的先天性炎症激活、糖原积累和感染性降低。基因距离越远的C.豚鼠GPIC是豚鼠的天然病原体，当阴道内接种时诱导上生殖道病理，模拟人类疾病。为了研究pCpGP 1对C.本研究从GPIC菌株CC 13中分离出一株致病性好的菌株，并对其进行了体外和体内实验研究。CC 13的转录谱显示，在C.开玩笑。然而，GPIC和CC 13的2-脱氧葡萄糖（2DG）处理导致所有鉴定的PRCL（包括glgA）的转录减少，表明该物种中存在质粒非依赖性葡萄糖应答。与质粒固化的C. muridarum和C.沙眼衣原体、质粒治愈C.与野生型C.开玩笑。此外，由CC 13感染豚鼠诱导的炎症病理学与由GPIC诱导的炎症病理学相似，尽管我们在雌激素处理的豚鼠中观察到CC 13感染更快的消退。这些数据表明，质粒不参与表达或调节C.或者冗余效应子阻止了在C.卡那霉素质粒治愈菌株。
Loss of the conserved “cryptic” plasmid from C. trachomatis and C. muridarum is pleiotropic, resulting in reduced innate inflammatory activation via TLR2, glycogen accumulation and infectivity. The more genetically distant C. caviae GPIC is a natural pathogen of guinea pigs and induces upper genital tract pathology when inoculated intravaginally, modeling human disease. To examine the contribution of pCpGP1 to C. caviae pathogenesis, a cured derivative of GPIC, strain CC13, was derived and evaluated in vitro and in vivo. Transcriptional profiling of CC13 revealed only partial conservation of previously identified plasmid-responsive chromosomal loci (PRCL) in C. caviae. However, 2-deoxyglucose (2DG) treatment of GPIC and CC13 resulted in reduced transcription of all identified PRCL, including glgA, indicating the presence of a plasmid-independent glucose response in this species. In contrast to plasmid-cured C. muridarum and C. trachomatis, plasmid-cured C. caviae strain CC13 signaled via TLR2 in vitro and elicited cytokine production in vivo similar to wild-type C. caviae. Furthermore, inflammatory pathology induced by infection of guinea pigs with CC13 was similar to that induced by GPIC, although we observed more rapid resolution of CC13 infection in estrogen-treated guinea pigs. These data indicate that either the plasmid is not involved in expression or regulation of virulence in C. caviae or that redundant effectors prevent these phenotypic changes from being observed in C. caviae plasmid-cured strains.
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影响因子：
4.4
作者：
Mitchell CM;Hovis KM;Bavoil PM;Myers GS;Carrasco JA;Timms P
通讯作者：
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影响因子：
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发表时间：
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