Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species.
Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species.
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DOI：
10.1186/1471-2164-11-442
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发表时间：
2010-07-21
期刊：
影响因子：
4.4
通讯作者：
Timms P
中科院分区：
文献类型：
--
作者：
Mitchell CM;Hovis KM;Bavoil PM;Myers GS;Carrasco JA;Timms P
肺炎衣原体是一种广泛存在的病原体，除了引起人类和动物的一系列其他疾病外，还引起上呼吸道和下呼吸道感染。以前的全基因组分析集中在四个基本上克隆的（> 99%的同一性）C。pneumoniae人基因组（AR 39、CWL 029、J138和TW 183）的基因组序列，对该物种的菌株多样性和进化提供了相对较少的了解。我们对最近测序的C. pneumoniae koala基因组和4个C.肺炎人类基因组鉴定物种特异性基因，更重要的是，以获得对物种的遗传多样性和进化的洞察。我们选择了分散在整个染色体上的基因，代表了C。肺炎杆菌，在衣原体生物学和/或致病性中具有已证实作用的基因（n = 49），编码核苷酸补救或氨基酸生物合成蛋白的基因（n = 6），以及染色体外元件（9个质粒和2个噬菌体基因）。我们已经确定了菌株特异性差异和检测C的目标。来自人和动物来源的肺炎分离物。这种特征对于更好地理解疾病传播和干预是必要的。
Chlamydia pneumoniae is a widespread pathogen causing upper and lower respiratory tract infections in addition to a range of other diseases in humans and animals. Previous whole genome analyses have focused on four essentially clonal (> 99% identity) C. pneumoniae human genomes (AR39, CWL029, J138 and TW183), providing relatively little insight into strain diversity and evolution of this species. We performed individual gene-by-gene comparisons of the recently sequenced C. pneumoniae koala genome and four C. pneumoniae human genomes to identify species-specific genes, and more importantly, to gain an insight into the genetic diversity and evolution of the species. We selected genes dispersed throughout the chromosome, representing genes that were specific to C. pneumoniae, genes with a demonstrated role in chlamydial biology and/or pathogenicity (n = 49), genes encoding nucleotide salvage or amino acid biosynthesis proteins (n = 6), and extrachromosomal elements (9 plasmid and 2 bacteriophage genes). We have identified strain-specific differences and targets for detection of C. pneumoniae isolates from both human and animal origin. Such characterisation is necessary for an improved understanding of disease transmission and intervention.
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