Centrosomal kinases, HsAIRK1 and HsAIRK3, are overexpressed in primary colorectal cancers.
Centrosomal kinases, HsAIRK1 and HsAIRK3, are overexpressed in primary colorectal cancers.
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DOI：
10.1111/j.1349-7006.2000.tb00878.x
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发表时间：
2000-10
期刊：
影响因子：
--
通讯作者：
Saji S
中科院分区：
文献类型：
--
作者：
Takahashi T;Futamura M;Yoshimi N;Sano J;Katada M;Takagi Y;Kimura M;Yoshioka T;Okano Y;Saji S
新近发现的智人极光/Ipl1相关蛋白(HsAIRKs)家族成员，与染色体分离蛋白、苍蝇Aurora和酵母Ipl1同源，在M期高表达，并被认为调节中心体功能、染色体分离和细胞质分裂。本研究采用免疫组织化学方法检测了78例原发性结直肠癌、36例结直肠腺瘤和15例正常大肠组织中HsAIRK1和HsAIRK3的表达。在15例正常结肠粘膜中，HsAIRK1和HsAIRK3分别有10例和12例隐窝细胞呈弱阳性，其余均为阴性。53例(67.9%)HsAIRK1和40例(51.3%)HsAIRK3表达升高。结直肠腺瘤中HsAIRK1和HsAIRK3的高表达分别为11例(30.6%)和7例(19.4%)，介于结直肠癌和正常大肠黏膜之间。有趣的是，HsAIRK1的过表达与结直肠癌的原发肿瘤侵袭(PT)和P53的积聚显著相关。增殖细胞核抗原标记指数(PCNALI)与这些蛋白的表达水平无明显相关性。结果提示，HsAIRK1和HsAIRK3的过表达可能参与了结直肠癌的发生和发展。
Members of the recently identified family of Homo sapiens Aurora/Ipl1‐related kinases (HsAIRKs), homologous to chromosome segregation kinases, fly Aurora and yeast Ipl1, are highly expressed during M phase, and have been suggested to regulate centrosome function, chromosome segregation, and cytokinesis. In the present study, immunohistochemical analyses were performed of HsAIRK1 and HsAIRK3 expression in 78 primary colorectal cancers and 36 colorectal adenomas as well as 15 normal colorectal specimens. In normal colon mucosa, some crypt cells showed weak positive staining in 10 and 12 out of 15 cases for HsAIRK1 and HsAIRK3, respectively, the remaining cases being negative. Elevated expression of HsAIRK1 was observed in 53 (67.9%) of the colorectal cancers, and of HsAIRK3 in 40 (51.3%). Furthermore, colorectal adenomas showed high expression of HsAIRK1 and HsAIRK3 in 11 (30.6%) and 7 (19.4%) cases, respectively, thus being intermediate between colorectal cancers and normal colorectal mucosa. Interestingly, HsAIRK1 overexpression was significantly associated with pT (primary tumor invasion) and p53 accumulation in colorectal cancers. There was no significant correlation between proliferating cell nuclear antigen‐labeling index (PCNA‐LI) and the levels of these proteins. The results suggest that overexpression of HsAIRK1 and HsAIRK3 might be involved in tumorigenesis and/or progression of colorectal cancers.
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影响因子：
4.8
作者：
Kimura, M;Matsuda, Y;Okano, Y
通讯作者：
Okano, Y
DOI：
10.1073/pnas.95.6.2950
发表时间：
1998-03-17
影响因子：
11.1
作者：
Lingle, WL;Lutz, WH;Salisbury, JL
通讯作者：
Salisbury, JL
影响因子：
8
作者：
Carroll, PE;Okuda, M;Fukasawa, K
通讯作者：
Fukasawa, K
影响因子：
4.4
作者：
Bernard, M;Sanseau, P;Prigent, C
通讯作者：
Prigent, C
影响因子：
56.9
作者：
Fukasawa, K;Choi, T;VandeVoude, GF
通讯作者：
VandeVoude, GF