Apoptotic Cell Death and Regeneration in the Newborn Retina After Irradiation Prior to Bone Marrow Transplantation
Apoptotic Cell Death and Regeneration in the Newborn Retina After Irradiation Prior to Bone Marrow Transplantation
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骨髓移植前照射后新生儿视网膜的凋亡细胞死亡和再生
DOI：
10.1080/02713680701389333
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发表时间：
2007
影响因子：
2
通讯作者：
T. Shimada
中科院分区：
文献类型：
--
作者：
T. Igarashi;K. Miyake;J. Hayakawa;Ken Kawabata;M. Ishizaki;Hiroshi Takahashi;T. Shimada
目的：我们研究了循环骨髓（BM）来源的细胞对BM移植小鼠新生和成熟视网膜的贡献。研究方法：新生和成年C57 BL/6 J小鼠给予致死剂量的全身照射，之后定期评估视网膜的病理变化。此外，小鼠接受来自8周龄绿色荧光蛋白（GFP）转基因小鼠的BM细胞，并研究GFP+细胞的后续分化。结果：照射后5小时内，新生小鼠视网膜细胞开始凋亡。相比之下，成年小鼠的照射没有引起视网膜的组织学变化。BM细胞在成年小鼠中通常不分化，但许多GFP+ BM细胞整合到新生小鼠的视网膜组织中，在那里它们表达各种细胞类型特异性标记。最后，检查整个视网膜安装显示，GFP+细胞也有助于视网膜血管化。结论：我们的研究结果强调了在利用骨髓移植的模型中仔细评估辐射的生物学效应的重要性。
Purpose: We studied the contribution made by circulating bone marrow (BM)-derived cells to the newborn and mature retinas of BM-transplanted mice. Methods: Newborn and adult C57BL/6J mice were administered a lethal dose of total-body irradiation, after which pathologic changes to the retinas were periodically assessed. In addition, mice received BM cells from 8-week-old green fluorescent protein (GFP) transgenic mice, and the subsequent differentiation of GFP+ cells was studied. Results: Within 5 hr after irradiation of newborn mice, retinal cells began to die due to apoptosis. By contrast, irradiation of adult mice elicited no histologic changes in the retina. BM cells generally did not differentiate in adult mice, but numerous GFP+ BM cells were integrated into the retinal tissue of newborn mice, where they expressed various cell type–specific markers. Finally, examination of whole retina mounts showed that GFP+ cells also contributed to retinal vascularization. Conclusions: Our findings underscore the importance of careful evaluation of the biological effects of irradiation in models making use of BM transplantation.
影响因子：
56.9
作者：
Brazelton, TR;Rossi, FMV;Blau, HM
通讯作者：
Blau, HM
影响因子：
56.9
作者：
Mezey, É;Chandross, KJ;McKercher, SR
通讯作者：
McKercher, SR
影响因子：
20.1
作者：
Asahara, T;Masuda, H;Isner, JM
通讯作者：
Isner, JM