Detection of Antibodies to Ehrlichia spp. in Dromedary Camels and Co-Grazing Sheep in Northern Kenya Using an Ehrlichia ruminantium Polyclonal Competitive ELISA.
Detection of Antibodies to Ehrlichia spp. in Dromedary Camels and Co-Grazing Sheep in Northern Kenya Using an Ehrlichia ruminantium Polyclonal Competitive ELISA.
复制标题
DOI：
10.3390/microorganisms10050916
复制
发表时间：
2022-04-27
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
一种临床和死后表现与在心脏病中观察到的疾病相似的疾病，是由细胞内细菌Ehrlichia ruminantium引起的家养和野生反刍动物的蜱传疾病，于2016年首次在肯尼亚的单峰骆驼中报告;当时进行的调查确定了原因，但没有定论。在本研究中，我们筛选了2016年疾病爆发之前（2015年）和之后（2020年）收集的肯尼亚骆驼血清中的埃立克体抗体。使用E.反刍动物多克隆竞争ELISA（PC-ELISA）。对于2015年和2020年采样的动物，来自报告心水样疾病地区的骆驼的中位抗体水平显著高于来自无疾病地区的骆驼（p < 0.0001）。2015年采样的骆驼的总体血清阳性中位数高于2020年，这可能是由于前一组的平均年龄较高。PCR阳性的骆驼Ehrlichia regneryi显着低于（p = 0.03）的中位数抗体水平比PCR阴性骆驼。我们的研究结果表明，肯尼亚骆驼经常暴露于E。ruminantium，E.反刍动物是不可能的唯一原因爆发的心脏水样疾病;和钙。E. regneryi与E. PC-ELISA中的反刍动物。
A disease with clinical and post-mortem presentation similar to those seen in heartwater, a tick-borne disease of domestic and wild ruminants caused by the intracellular bacterium Ehrlichia ruminantium, was first reported in dromedary camels in Kenya in 2016; investigations carried out at the time to determine the cause were inconclusive. In the present study, we screened sera from Kenyan camels collected before (2015) and after (2020) the 2016 disease outbreak for antibodies to Ehrlichia spp. using an E. ruminantium polyclonal competitive ELISA (PC-ELISA). Median antibody levels were significantly higher (p < 0.0001) amongst camels originating from areas where the heartwater-like disease was reported than from disease-free areas, for animals sampled in both 2015 and 2020. Overall median seropositivity was higher in camels sampled in 2015 than in 2020, which could have been due to higher mean age in the former group. Camels that were PCR-positive for Candidatus Ehrlichia regneryi had significantly lower (p = 0.03) median antibody levels than PCR-negative camels. Our results indicate that Kenyan camels are frequently exposed to E. ruminantium from an early age, E. ruminantium was unlikely to have been the sole cause of the outbreak of heartwater-like disease; and Ca. E. regneryi does not appreciably cross-react with E. ruminantium in the PC-ELISA.
登录
查看更多内容
DOI：
10.1099/ijsem.0.000895
发表时间：
2016-03-01
影响因子：
2.8
作者：
Cabezas-Cruz, Alejandro;Zweygarth, Erich;de la Fuente, Jose
通讯作者：
de la Fuente, Jose
影响因子：
2.2
作者：
Mattioli, RC;Bah, M;Jongejan, F
通讯作者：
Jongejan, F
影响因子：
2.6
作者：
Awa, DN
通讯作者：
Awa, DN
影响因子：
0.8
作者：
AZWAI, SM;CARTER, SD;WOLDEHIWET, Z
通讯作者：
WOLDEHIWET, Z
影响因子：
2.4
作者：
Alsubki RA;Albohairy FM;Attia KA;Kimiko I;Selim A;Sayed-Ahmed MZ
通讯作者：
Sayed-Ahmed MZ