Insulin receptor binding and protein kinase activity in muscles of trained rats.
Insulin receptor binding and protein kinase activity in muscles of trained rats.
复制标题
受过训练的大鼠肌肉中的胰岛素受体结合和蛋白激酶活性。
作者：
G. Dohm;M. Sinha;J. Caro
运动已被证明可以增加胰岛素敏感性，肌肉是胰岛素作用的最重要组织。由于胰岛素作用的第一步是与膜受体结合，我们假设运动训练会改变肌肉中的胰岛素受体，在本研究中，我们对这一假设进行了研究。最初体重约为100 g的雌性大鼠在25 m/min（0级）的跑步机上跑步2 h/天，6天/周，持续4周。将来自股中间肌的胰岛素受体用含有1%Triton X-100的缓冲液溶解，然后将可溶性提取物通过麦胚凝集素柱进行部分纯化。4周的训练方案导致红色股外侧肌中柠檬酸合酶活性增加65%，表明对运动的适应。在所有胰岛素浓度下，部分纯化的受体制剂在训练大鼠肌肉中的胰岛素结合率约为两倍，表明受体数量增加。在还原和非还原条件下的电泳迁移率证明，训练并没有改变胰岛素受体的结构。基础胰岛素受体蛋白激酶活性在训练的动物中高于未训练的动物，这可能是由于受体数量较多。然而，胰岛素刺激的蛋白激酶活性被抑制的训练。这些结果表明，耐力训练确实改变了肌肉中受体的数量和功能，这些变化可能在运动训练后增加胰岛素敏感性方面很重要。
Exercise has been shown to increase insulin sensitivity, and muscle is quantitatively the most important tissue of insulin action. Since the first step in insulin action is the binding to a membrane receptor, we postulated that exercise training would change insulin receptors in muscle and in this study we have investigated this hypothesis. Female rats initially weighing approximately 100 g were trained by treadmill running for 2 h/day, 6 days/wk for 4 wk at 25 m/min (0 grade). Insulin receptors from vastus intermedius muscles were solubilized by homogenizing in a buffer containing 1% Triton X-100 and then partially purified by passing the soluble extract over a wheat germ agglutinin column. The 4 wk training regimen resulted in a 65% increase in citrate synthase activity in red vastus lateralis muscle, indicating an adaptation to exercise. Insulin binding by the partially purified receptor preparation s was approximately doubled in muscle of trained rats at all insulin concentrations, suggesting an increase in the number of receptors. Training did not alter insulin receptor structure as evidenced by electrophoretic mobility under reducing and nonreducing conditions. Basal insulin receptor protein kinase activity was higher in trained than untrained animals and this was likely due to the greater number of receptors. However, insulin stimulation of the protein kinase activity was depressed by training. These results demonstrate that endurance training does alter receptor number and function in muscle and these changes may be important in increasing insulin sensitivity after exercise training.
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影响因子：
3.3
作者：
CRAIG, BW;HAMMONS, GT;HOLLOSZY, JO
通讯作者：
HOLLOSZY, JO
影响因子：
15.9
作者：
RICHTER, EA;GARETTO, LP;RUDERMAN, NB
通讯作者：
RUDERMAN, NB
年轻和老年运动员以及久坐男性的葡萄糖耐量。
DOI：
10.1152/jappl.1984.56.6.1521
发表时间：
1984
期刊：
Journal of applied physiology: respiratory, environmental and exercise physiology
影响因子：
--
作者：
Seals,DR;Hagberg,JM;Allen,WK;Hurley,BF;Dalsky,GP;Ehsani,AA;Holloszy,JO
通讯作者：
Holloszy,JO
胰岛素受体的β亚基是胰岛素激活的蛋白激酶。
影响因子：
2.9
作者：
Shia,MA;Pilch,PF
通讯作者：
Pilch,PF
影响因子：
3.9
作者：
Dubler,RE;Whipple,JH;Larner,J
通讯作者：
Larner,J