Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development.
Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development.
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DOI：
10.1002/dvdy.24126
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发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Conlon FL
中科院分区：
文献类型：
--
作者：
Amin NM;Gibbs D;Conlon FL
锌指转录因子CASZ1是发育过程中分化成不同类型心肌细胞所必需的。然而，CASZ1在整个心脏发育过程中都有表达，提示CASZ1的空间调控发生在蛋白质水平。对CASZ1在心脏中的转录后调控知之甚少。我们产生了在非洲爪哇胚胎发育中特异性识别CASZ1的抗体，并在心脏发育期间对CASZ1进行了免疫荧光分析。CASZ1在整个发育心肌中均有表达。CASZ1仅限于终末分化的心肌细胞，在重新进入细胞周期的细胞中下调。我们确定CASZ1的表达与心肌细胞、骨骼肌细胞和淋巴-心脏肌肉系统的终末分化有关。本研究表明，CASZ1蛋白的空间差异表达可能是由于心脏发育过程中CASZ1基因转录后调控所致。这项研究的结果为了解CASZ1在心脏功能和其他类型细胞分化中的作用提供了深入的见解，包括骨骼肌和淋巴心。
The zinc-finger transcription factor CASZ1 is required for differentiation of a distinct population of cardiomyocytes during development. However, expression of Casz1 mRNA is detected throughout the developing heart, suggesting the spatial regulation of CASZ1 occurs at the protein level. Relatively little is known about posttranscriptional regulation of Casz1 in the heart. We generated antibodies that specifically recognize CASZ1 in developing Xenopus embryos, and performed immunofluorescence analysis of CASZ1 during cardiac development. CASZ1 was detected throughout the developing myocardium. CASZ1 was restricted to terminally differentiated cardiomyocytes, and was down-regulated in cells that re-enter the cell cycle. We determined that CASZ1 expression correlated with terminal differentiation in cardiac muscle cells, skeletal muscle cells, and lymph-heart musculature. This study indicates that spatially distinct expression of CASZ1 protein may be due to posttranscriptional control of Casz1 mRNA during cardiac development. The results of this study provide insights into the role of Casz1 in cardiac function and in the differentiation of other cell types, including skeletal muscle and lymph heart.
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