Plasmodium chabaudi limits early Nippostrongylus brasiliensis-induced pulmonary immune activation and Th2 polarization in co-infected mice.
Plasmodium chabaudi limits early Nippostrongylus brasiliensis-induced pulmonary immune activation and Th2 polarization in co-infected mice.
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DOI：
10.1186/1471-2172-10-60
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发表时间：
2009-12-01
期刊：
影响因子：
3
通讯作者：
Graham AL
中科院分区：
文献类型：
--
作者：
Hoeve MA;Mylonas KJ;Fairlie-Clarke KJ;Mahajan SM;Allen JE;Graham AL
几种常见寄生线虫的幼虫专性地通过宿主肺迁移，并经常损害宿主肺。幼虫诱导强烈的肺2型免疫应答，包括辅助性T细胞（Th）2以及交替激活的巨噬细胞（AAMφ）和相关的几丁质酶和Fizz/Fizn家族成员（ChaFF），其被认为促进组织修复过程。鉴于系统性或肺部居民1型诱导病原体在线虫流行的地理区域中的流行，我们希望调查并发1型反应对线虫幼虫迁移的2型反应的发展的影响。因此，我们感染BALB/c小鼠与线虫Nippostrongylus巴西，在存在或不存在的疟原虫chabaudi chabaudi疟疾寄生虫。共感染的动物在同一天接受两种感染，每天评估疾病，然后在感染后3、5、7或20天进行免疫学测量。我们观察到，线虫本身造成短暂的体重和红细胞密度的损失，但合并感染，然后轻微改善疟疾贫血的严重程度。我们还跟踪了肺和胸淋巴结中免疫反应的发展。在感染后7天左右适应性免疫应答开始时，疟疾共感染减少了ChaFF的肺部表达。对T细胞应答的评估表明，疟疾共感染也显著损害了对线虫的Th 2应答。P. C. chabaudi共感染改变了局部和淋巴结2型免疫活化，这是由于N.巴西幼虫考虑到其他实验室最近的工作表明，N。巴西线虫诱导的ChaFF与长期肺损伤的程度相关，我们的研究结果提出了与疟疾共感染可能改变线虫迁移后肺修复过程的可能性。在这里开发的混合感染模型中的进一步实验将揭示肺中疟疾和蠕虫同时存在的长期后果。
Larvae of several common species of parasitic nematodes obligately migrate through, and often damage, host lungs. The larvae induce strong pulmonary Type 2 immune responses, including T-helper (Th)2 cells as well as alternatively activated macrophages (AAMφ) and associated chitinase and Fizz/resistin family members (ChaFFs), which are thought to promote tissue repair processes. Given the prevalence of systemic or lung-resident Type 1-inducing pathogens in geographical areas in which nematodes are endemic, we wished to investigate the impact of concurrent Type 1 responses on the development of these Type 2 responses to nematode larval migration. We therefore infected BALB/c mice with the nematode Nippostrongylus brasiliensis, in the presence or absence of Plasmodium chabaudi chabaudi malaria parasites. Co-infected animals received both infections on the same day, and disease was assessed daily before immunological measurements were taken at 3, 5, 7 or 20 days post-infection. We observed that the nematodes themselves caused transient loss of body mass and red blood cell density, but co-infection then slightly ameliorated the severity of malarial anaemia. We also tracked the development of immune responses in the lung and thoracic lymph node. By the time of onset of the adaptive immune response around 7 days post-infection, malaria co-infection had reduced pulmonary expression of ChaFFs. Assessment of the T cell response demonstrated that the Th2 response to the nematode was also significantly impaired by malaria co-infection. P. c. chabaudi co-infection altered both local and lymph node Type 2 immune activation due to migration of N. brasiliensis larvae. Given recent work from other laboratories showing that N. brasiliensis-induced ChaFFs correlate to the extent of long-term lung damage, our results raise the possibility that co-infection with malaria might alter pulmonary repair processes following nematode migration. Further experimentation in the co-infection model developed here will reveal the longer-term consequences of the presence of both malaria and helminths in the lung.
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影响因子：
6.7
作者：
Baer K;Klotz C;Kappe SH;Schnieder T;Frevert U
通讯作者：
Frevert U
影响因子：
11.4
作者：
Holcomb, IN;Kabakoff, RC;Hébert, CC
通讯作者：
Hébert, CC
影响因子：
3.1
作者：
Helmby, H;Kullberg, M;Troye-Blomberg, M
通讯作者：
Troye-Blomberg, M
影响因子：
2.1
作者：
Lawrence, RA;Gray, CA;Maizels, RM
通讯作者：
Maizels, RM
DOI：
10.1073/pnas.0707221105
发表时间：
2008-01-15
影响因子：
11.1
作者：
Graham, Andrea L.
通讯作者：
Graham, Andrea L.