Functional characterization of Lilium lancifolium cold-responsive Zinc Finger Homeodomain (ZFHD) gene in abscisic acid and osmotic stress tolerance.
Functional characterization of Lilium lancifolium cold-responsive Zinc Finger Homeodomain (ZFHD) gene in abscisic acid and osmotic stress tolerance.
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百合冷响应锌指同源域（ZFHD）基因在脱落酸和渗透胁迫耐受中的功能特征
作者：
Yong Y;Zhang Y;Lyu Y
我们之前通过基因共表达网络鉴定对虎百合（Lilium lancifolium）成熟叶片的冷响应转录组进行了分析。结果表明，一个编码锌指同源结构域蛋白的ZFHD基因可能在虎百合对冷胁迫的响应中起重要的调控作用。本研究进一步研究了ZFHD基因（称为LlZFHD4）对渗透胁迫的反应，包括冷、盐、水胁迫和脱落酸（ABA）。根据转录组序列，从虎百合成熟叶片中克隆出LlZFHD4的编码区和5′启动子区。在连续4°C、NaCl、PEG和ABA处理下进行应力响应分析。在转基因拟南芥、烟草和酵母中对LlZFHD4进行了功能表征。LlZFHD4编码一种由180个氨基酸组成的核定位蛋白。LlZFHD4的n端区域在酵母中具有转录激活活性。4°C、NaCl、PEG和ABA处理均诱导LlZFHD4的表达。几种应激或激素反应的顺式作用调控元件（T-Box, BoxI）。在LlZFHD4核心启动子区（789 bp）发现了转录因子MYC、DRE和W-box的结合位点。此外，由LlZFHD4启动子驱动的GUS基因在低温、NaCl、水分胁迫和ABA胁迫下均上调表达。过表达LlZFHD4提高转基因拟南芥的耐旱性和耐寒性在4°C和PEG条件下，与野生型（WT）相比，LlZFHD4转基因植株的成活率更高，渗透调节能力更强。然而，与WT植株相比，LlZFHD4转基因植株对盐的耐受性较低，对ABA的敏感性较高。与野生型相比，LlZFHD4转基因拟南芥中胁迫和ABA应答基因的转录水平明显上调，表明LlZFHD4参与ABA信号通路，在虎百合对冷、盐和水胁迫的应答中起重要作用。
We have previously performed an analysis of the cold-responsive transcriptome in the mature leaves of tiger lily (Lilium lancifolium) by gene co-expression network identification. The results has revealed that a ZFHD gene, notated as encoding zinc finger homeodomain protein, may play an essential regulating role in tiger lily response to cold stress. A further investigation of the ZFHD gene (termed as LlZFHD4) responding to osmotic stresses, including cold, salt, water stresses, and abscisic acid (ABA) was performed in this study. Based on the transcriptome sequences, the coding region and 5′ promoter region of LlZFHD4 were cloned from mature tiger lily leaves. Stress response analysis was performed under continuous 4 °C, NaCl, PEG, and ABA treatments. Functional characterization of LlZFHD4 was conducted in transgenic Arabidopsis, tobacco, and yeast. LlZFHD4 encodes a nuclear-localized protein consisting of 180 amino acids. The N-terminal region of LlZFHD4 has transcriptional activation activity in yeast. The 4 °C, NaCl, PEG, and ABA treatments induced the expression of LlZFHD4. Several stress- or hormone-responsive cis-acting regulatory elements (T-Box, BoxI. and ARF) and binding sites of transcription factors (MYC, DRE and W-box) were found in the core promoter region (789 bp) of LlZFHD4. Also, the GUS gene driven by LlZFHD4 promoter was up-regulated by cold, NaCl, water stresses, and ABA in Arabidopsis. Overexpression of LlZFHD4 improved cold and drought tolerance in transgenic Arabidopsis; higher survival rate and better osmotic adjustment capacity were observed in LlZFHD4 transgenic plants compared to wild type (WT) plants under 4 °C and PEG conditions. However, LlZFHD4 transgenic plants were less tolerant to salinity and more hypersensitive to ABA compared to WT plants. The transcript levels of stress- and ABA-responsive genes were much more up-regulated in LlZFHD4 transgenic Arabidopsis than WT. These results indicate LlZFHD4 is involved in ABA signaling pathway and plays a crucial role in regulating the response of tiger lily to cold, salt and water stresses.
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影响因子：
4.4
作者：
Khatun K;Nath UK;Robin AHK;Park JI;Lee DJ;Kim MB;Kim CK;Lim KB;Nou IS;Chung MY
通讯作者：
Chung MY
DOI：
10.1073/pnas.1718099115
发表时间：
2018-05-08
影响因子：
11.1
作者：
Perrella G;Davidson MLH;O'Donnell L;Nastase AM;Herzyk P;Breton G;Pruneda-Paz JL;Kay SA;Chory J;Kaiserli E
通讯作者：
Kaiserli E
影响因子：
7.4
作者：
Sakamoto, H;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
3.7
作者：
Sun, Jinhao;Xie, Minmin;Sun, Yuhe
通讯作者：
Sun, Yuhe
影响因子：
7.4
作者：
Cham Thi Tuyet Le;Brumbarova, Tzvetina;Bauer, Petra
通讯作者：
Bauer, Petra