ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in Arabidopsis.
ZINC-FINGER interactions mediate transcriptional regulation of hypocotyl growth in Arabidopsis.
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锌指相互作用介导拟南芥下胚轴生长的转录调控。
DOI：
10.1073/pnas.1718099115
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发表时间：
2018-05-08
影响因子：
11.1
通讯作者：
Kaiserli E
中科院分区：
文献类型：
--
作者：
Perrella G;Davidson MLH;O'Donnell L;Nastase AM;Herzyk P;Breton G;Pruneda-Paz JL;Kay SA;Chory J;Kaiserli E
光在植物发育过程中协调能量的产生、生长和存活。在拟南芥中，光通过光感受器、转录因子和信号元件的作用，在光形态建成和开花等发育转变过程中刺激转录重编程。在这里，我们指定了锌指同源域(ZFHD)转录因子家族的一个成员在调节光诱导发育中的功能。我们的发现揭示了ZFHD10在理解最近发现的光和光周期开花的整合因子串联锌指PLUS3(TZP)如何通过与光调控的启动子元件直接相关来控制促进生长的转录调控因子的表达方面缺失了一环。阐明这些新的蛋白质复合体是如何协调基因表达的，将使科学家和育种者能够优化植物的生长和发育，以应对不利的环境条件。环境信号的整合以及光感受器和转录调控因子之间的相互作用是塑造植物发育的关键。串联锌指基因PLUS3(TZP)是光和光周期信号的整合因子，促进拟南芥开花。在这里，我们阐明了TZP作为下胚轴伸长的正调节因子的分子作用。我们确定了TZP的相互作用伙伴，转录因子锌指同源结构域10(ZFHD10)，并研究了其在协同调节蓝光依赖的转录调控基因和促生长基因表达方面的功能。通过采用全基因组的方法，我们揭示了ZFHD10和TZP与富含光调控元件的启动子靶标相互作用。此外，采用有针对性的方法，我们发现ZFHD10招募TZP为关键共调节基因的启动子。我们的发现不仅揭示了TZP在转录水平上促进下胚轴伸长的作用机制，而且还赋予了ZFHD转录因子家族中一个未知的成员促进植物生长的功能。
Light coordinates energy production, growth, and survival throughout plant development. In Arabidopsis, light stimulates transcriptional reprogramming during developmental transitions such as photomorphogenesis and flowering through the action of photoreceptors, transcription factors, and signaling components. Here we assign a function to a member of the zinc-finger homeodomain (ZFHD) transcription factor family in regulating light-induced development. Our findings reveal ZFHD10 to be a missing link in understanding how the recently discovered integrator of light and photoperiodic flowering, TANDEM ZINC-FINGER PLUS3 (TZP), controls the expression of growth-promoting transcriptional regulators via direct association with light-regulated promoter elements. Elucidating how such novel protein complexes coordinate gene expression will allow scientists and breeders to optimize plant growth and development in response to unfavorable environmental conditions. Integration of environmental signals and interactions among photoreceptors and transcriptional regulators is key in shaping plant development. TANDEM ZINC-FINGER PLUS3 (TZP) is an integrator of light and photoperiodic signaling that promotes flowering in Arabidopsis thaliana. Here we elucidate the molecular role of TZP as a positive regulator of hypocotyl elongation. We identify an interacting partner for TZP, the transcription factor ZINC-FINGER HOMEODOMAIN 10 (ZFHD10), and characterize its function in coregulating the expression of blue-light–dependent transcriptional regulators and growth-promoting genes. By employing a genome-wide approach, we reveal that ZFHD10 and TZP coassociate with promoter targets enriched in light-regulated elements. Furthermore, using a targeted approach, we show that ZFHD10 recruits TZP to the promoters of key coregulated genes. Our findings not only unveil the mechanism of TZP action in promoting hypocotyl elongation at the transcriptional level but also assign a function to an uncharacterized member of the ZFHD transcription factor family in promoting plant growth.
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影响因子：
11.6
作者：
Kohnen, Markus V.;Schmid-Siegert, Emanuel;Fankhauser, Christian
通讯作者：
Fankhauser, Christian
影响因子：
18
作者：
Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
通讯作者：
Wigge PA
影响因子：
7.2
作者：
Bowler, C;Benvenuto, G;Paszkowski, J
通讯作者：
Paszkowski, J
影响因子：
9.2
作者：
de Wit, Mieke;Keuskamp, Diederik H.;Pierik, Ronald
通讯作者：
Pierik, Ronald
影响因子：
11.4
作者：
Hu, Wei;dePamphilis, Claude W.;Ma, Hong
通讯作者：
Ma, Hong