Identification of IS 1206, a Corynebacterium glutamicum IS3‐related insertion sequence and phylogenetic analysis
Identification of IS 1206, a Corynebacterium glutamicum IS3‐related insertion sequence and phylogenetic analysis
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谷氨酸棒杆菌 IS3 相关插入序列 IS 1206 的鉴定和系统发育分析
DOI:
10.1111/j.1365-2958.1994.tb02190.x
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发表时间:
1994
影响因子:
3.6
通讯作者:
G. Leblon
中科院分区:
文献类型:
--
作者:
C. Bonamy;J. Labarre;O. Reyes;G. Leblon
Integration of plasmid pCGL320 into a Corynebacterium glutamicum ATCC21086 derivative led to tandem amplification of the inserted plasmid (Labarre et a/., 1993). One amplification event was associated with integration of an insertion sequence that we have named IS 1206. Hybridizing sequences were only found in C. glutamicum strains and at various copy numbers. IS1206 is 1290bp long, carries 32 bp imperfect inverted repeats and generates a 3bp duplication of the target DNA upon insertion. IS1206 presents the features characteristic of the IS3 family and part of the DNA sequence centering on the putative transposase region (orfB) is similar to those of IS3 and some other related elements. Phylogenetic analysis of orfB deduced protein sequences from IS 1206 and IS3‐related elements contradicts the phylogeny of the species, suggesting that evolution of these elements might be complex. Horizontal transfer could be invoked but other alternatives like ancestral polymorphism or/and different rates of evolution could also be involved.
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DOI:
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DOI:
--
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