Nanoscale Surface Topography Reduces Focal Adhesions and Cell Stiffness by Enhancing Integrin Endocytosis.
Nanoscale Surface Topography Reduces Focal Adhesions and Cell Stiffness by Enhancing Integrin Endocytosis.
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DOI：
10.1021/acs.nanolett.1c01934
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发表时间：
2021-10-13
期刊：
影响因子：
10.8
通讯作者：
Cui B
中科院分区：
文献类型：
--
作者：
Li X;Klausen LH;Zhang W;Jahed Z;Tsai CT;Li TL;Cui B
底物硬度和表面形貌都通过机械转导信号通路调节细胞行为。这种相互交织的影响表明，在生物医学应用中，工程化的表面形貌可能会取代或消除基质硬度的影响。然而，细胞识别地形特征的机制尚不完全清楚。在这里，我们证明了纳米拓扑学的存在极大地改变了细胞的行为，以至于在坚硬的玻璃基质上培养的神经元和干细胞的行为就像它们在软水凝胶上一样。利用原子力显微镜，我们发现刚性纳米拓扑术在降低细胞硬度和膜张力方面类似于软水凝胶的效果。此外，我们揭示，纳米拓扑术通过增强细胞吞噬作用和随后去除整合素受体来减少局部粘连和细胞僵硬。这种对机理的理解将支持纳米拓扑学的合理设计，这种设计指导刚性材料上的细胞表现得就像它们在软质材料上一样。
Both substrate stiffness and surface topography regulate cell behavior through mechanotransduction signaling pathways. Such intertwined effects suggest that engineered surface topographies might substitute or cancel the effects of substrate stiffness in biomedical applications. However, the mechanisms by which cells recognize topographical features are not fully understood. Here we demonstrate that the presence of nanotopography drastically alters cell behavior such that neurons and stem cells cultured on rigid glass substrates behave as if they were on soft hydrogels. With atomic force microscopy, we show that rigid nanotopography resembles the effects of soft hydrogels in reducing cell stiffness and membrane tension. Further, we reveal that nanotopography reduces focal adhesions and cell stiffness by enhancing the endocytosis and the subsequent removal of integrin receptors. This mechanistic understanding will support the rational design of nanotopography that directs cells on rigid materials to behave as if they were on soft ones.
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